巨噬细胞在婴幼儿血管瘤中分布及表型变化的研究

Distribution and phenotype changes of macrophage in infantile hemangioma

目的 观察婴幼儿血管瘤中巨噬细胞的分布及其表型变化过程.方法 收集手术切除的婴幼儿血管瘤标本43例,分别使用CD68、CD16/32、CD163标记非特异性巨噬细胞、M1和M2型巨噬细胞,免疫组化染色观察其在血管瘤组织中的分布及变化.结果 ①CD68阳性巨噬细胞在增生早期大量出现,聚集于婴幼儿血管瘤新生血管的周围;增生中期数量减少,弥散于瘤体中;增生晚期和消退早期,数量进一步减少,散落于瘤体中.②M2型巨噬细胞在小于3个月的血管瘤标本中表达明显,在大于3个月的标本中迅速减少.③M1型巨噬细胞在部分增生晚期和消退早期标本中表达明显.结论 巨噬细胞参与了婴幼儿血管瘤的增殖与消退过程;早期出现的巨噬细胞为M2型,在增殖中扮演重要角色;M1型巨噬细胞可能参与消退过程.

Objective To observe the distribution and phenotypc changes of macrophage in infantile hemangioma. Methods Forty-three infantile hemangioma samples were harvested from resection operation, Distribution and phenolype changes of macrophages labelled with CD68,CD16/32 and CD163 in infantile hemangioma were investigated by immunohistochemistry. Results CD68 positive maerophages were observed around infantile hemangioma at the early stage of proliferation, but the quantity decreased gradually at the middle and the late stages. CD163 positive maerophage was observed in tile early proliferating stage. CD16/32 positive macrophage can be observed in part of the late proliferating and early degenerating stage. Conclusion Maerophages may be involved in the whole process of proliferation and degeneration of infantile hemangioma. Type-2 macrophages play an important role in the early proliferating stage; type-1 macrophages may participate in the degenerating proeess.