蝉蜕活性成分的提取及其抑菌活性的研究

Analyses on ingredients and antibacterial activity of periostracum cicadae

本文报道了对传统中药蝉蜕抑菌活性成分提取与抑菌活性研究结果。对4种提取方法和7种提取溶剂的提取效果进行了平行比较研究,研究结果显示,以体积分数95%乙醇作为提取溶剂,4种提取方法中,以超声波法提取时的浸膏得率最高。采用超声波提取法时,在7种提取溶剂中,以95%乙醇提取浸膏的得率最高。抑菌试验结果表明,不同方法获得提取物与对照组相比对Escheridia coli均有明显的抑菌作用(P≤0.05),但不同提取方法得到的提取物之间,并无显著的抑菌活性差异。其中采用超声波提取法,以95%乙醇为提取溶剂时,所得提取物的抑菌活性最高,对E.coli的最小抑菌浓度为0.078 mg/mL。该研究结果是蝉蜕抑菌成分的首次报道。

The antimicrobial components of the traditional Chinese herbal medicine,periostrcum cicadae were extracted and their antibacterial activity examined. Four extraction methods and seven extraction solvents wereused. The results show that the highest yield was produced by ultrasonic extraction with 95% ethanol. The antibacterial activity of the obtained extracts against Escherichia coli was determined using different bioassays.The bioassay results indicate that all extracts had an obvious inhibitory effect on E. coli compared to the control（P≤0.05）. There was no significant difference in antimicrobial activity between different extracts. The highest antimicrobial activity was from extracts obtained by ultrasonic extraction with 95% ethanol,the minimum inhibitory concentration （MIC） of this extract on E.coli was 0.078 mg /mL.