催产素受体基因敲除对雌激素调节骨密度的影响

Effect of Oxtr knockout on estrogen regulating bone mineral density

目的:探讨催产素受体基因敲除对雌激素调节骨密度的影响。方法:选取3月龄野生型Oxtr+/+和纯合型Oxtr-/-雌性BABL/c小鼠各20只,随机分成野生型组(WT组)、野生型+雌二醇组(WT+E2组)、野生型去势组(WTOVX组)、野生型去势+雌二醇组(WTOVX+E2组)、纯合型组(KO组)、纯合型+雌二醇组(KO+E2组)、纯合型去势组(KO OVX组)及纯合型去势+雌二醇组(KO OVX+E2组),每组5只。17β-雌二醇皮下注射,每只50μg.d-1,每天1次。实验4周后测量各组小鼠脊柱和股骨的骨密度。结果:与WT组相比较,WT+E2组的骨密度显著增高(P<0.05),WTOVX组的骨密度显著降低(P<0.05),WTOVX+E2组的骨密度差异无统计学意义(P>0.05);与WTOVX组相比较,WTOVX+E2组的骨密度明显增高,差异具有统计学意义(P<0.05);与KO组相比较,KO+E2组、KO OVX组及KO OVX+E2组的骨密度差异均无统计学意义(P>0.05);KO OVX+E2组与KO OVX组相比较,骨密度差异也无统计学意义(P>0.05)。结论:催产素参与调节雌激素的骨合成代谢作用。

Objective： To explore the effect of Oxtr knockout on estrogen regulating bone mineral density（BMD）.Methods： Three-month old Oxtr＋/＋ and Oxtr-/-female BABL/c mice（20 mice per genotype） were randomly divided into wild type group（WT）,wild type ＋ estradiol group（WT＋E2）,wild type OVX group（WT OVX）,wild type OVX ＋ estradiol group（WT OVX＋E2）,Oxtr knockout group（KO）,Oxtr knockout ＋ estradiol group（KO＋E2）,Oxtr knockout OVX group（KO OVX） and Oxtr knockout OVX ＋ estradiol group（KO OVX＋E2）,5 mice per group.After injecting 17β-estradiol（50 μg·d-1,subcutaneous injection） for 4 weeks,spine and femur BMD of all group mice were measured.Results： BMD of WT OVX group mice were markedly lower than that of WT group mice.Injecting 17β-estradiol observably increased BMD of WT E2 group mice compared with WT group mice.Moreover,the decline in BMD of WT OVX mice was remarkably reversed by 17β-estradiol.But there was no significant difference on BMD between KO group and KO OVX group mice,and both of KO E2 group and KO OVX E2 group mice failed to respond to 17β-estradiol.Conclusion： Oxytocin is required for the anabolic action of estrogen on the skeleton.