脂蛋白脂酶基因HindⅢ和PvuⅡ多态性与子痫前期相关性研究

Association of the lipoprotein lipase Hind Ⅲ and Pvu Ⅱ polymorphisms with preeclampsia

目的:运用聚合酶链反应及限制性片段长度多态性(PCR-RFLP)方法研究脂蛋白脂酶(LPL)基因HindⅢ和PvuⅡ酶切位点多态性。从遗传学角度探讨其在子痫前期(PE)发生发展中的作用,并筛选易感基因,为PE的预防和治疗提供科学依据。方法:实验组按照乐杰主编《妇产科学》第6版标准,选取42例诊断子痫前期的患者,对照组选取50例年龄、孕周与实验组相近的正常孕妇。采用PCR-RFLP的方法,检测LPL基因PvuⅡ和HindⅢ酶切位点的多态性。结果:实验组LPL基因H+H+基因型33例(0.85),H+H-与H-H-基因型共6例(0.15),未出结果3例;对照组LPL基因H+H+基因型30例(0.60),H+H-与H-H-基因型共20例(0.40)。实验组等位基因H+71例(0.91),H-7例(0.09);对照组等位基因H+72例(0.72),H-28例(0.28)。基因型及等位基因分布频率组间比较,均P<0.05(基因型P=0.011,等位基因P=0.002),差异有统计学意义。LPL基因PvuⅡ酶切位点基因型(P+P+,P+P-,P-P-)及等位基因(P+,P-)分布频率在两组之间无明显差异(基因型P=0.627,等位基因P=0.941)。结论:LPL基因HindⅢ酶切位点多态性与PE的发生存在一定相关性,携带H+等位基因的孕妇可能更易发展成为PE。PvuⅡ酶切位点的基因多态性及等位基因P+与PE的发生无明显关联。

Objective： To study the relationship between lipoprotein lipase（LPL） gene Hind Ⅲ and Pvu Ⅱ polymorphism and the preeclampsia（PE） with polymerase chain reaction（PCR） and restriction fragment length polymorphism（RFLP） from the heredity perspective,screening the predisposing genes in order to provide a scientific base for preventing PE.Methods： LPL gene Pvu Ⅱ and Hind Ⅲ polymorphism were studied using PCR-RFLP in 42 PE and 50 healthy pregnant women with similar age and gestational weeks.Results： Results showed that 33 women in PE group had H＋H＋ and 6 women with H＋H-and H-H-genotype.The control group had 30 women with H＋H＋ and 20 women with H＋H-and H-H-genotype.The PE group had 71 allele of H＋ and 7 of H-,while the control group had 72 of H＋ and 28 of H-.There was a statistical significance both in the distribution of genotype and allelic frequency between the PE and control group（both the P0.05）,while the Pvu Ⅱ polymorphism between the PE and control group had no statistical significance both in distribution of genotype and allelic frequency（both the P0.05）.Conclusions： LPL gene Hind Ⅲ polymorphism is related with the occurance of PE.The H＋ carrier may be more likely to develop PE.While the Pvu Ⅱ polymorphism and allele P＋ have no significant relationship with PE.