胰腺内分泌肿瘤ghrelin和受体GHS-R1A的表达及患者血浆ghrelin、瘦素水平

Expression of ghrelin and its receptor GHS-R 1A in pancreatic endocrine tumors and the plasma levels of ghrelin and leptin

目的 检测胰腺内分泌肿瘤患者血浆及肿瘤组织ghrelin水平、血浆瘦素水平,探讨它们之间的关系及临床意义.方法 采用ELISA法检测11例胰腺内分泌肿瘤患者的术前血浆ghrelin及瘦素水平,以28例正常志愿者作为对照.免疫组织化学染色法检测11个肿瘤和27个对照组织ghrelin及其受体GHS-R1A的表达.并与临床病理资料进行相关分析.结果 胰腺内分泌肿瘤患者血浆ghrelin水平为（16.0±5.0）pg/ml,显著低于对照组的（21.0±2.0）pg/ml（P=0.047）;瘦素水平为（0.34±0.03）ng/ml,与对照组的（0.38±0.04）ng/ml无显著差异.肿瘤患者的血浆ghrelin与瘦素水平呈正相关（P=0.015）,但与各项临床病理指标均不相关;对照组的血浆瘦素水平与体重指数呈正相关（P=0.002）,而肿瘤患者两者不相关.肿瘤组织ghrelin的表达率明显低于对照组织（64%对100%,P=0.004）,而GHS-R1A的表达率与对照组无显著差异.肿瘤组织ghrelin和GHS-R 1A的表达与各项临床病理指标均不相关.结论 胰腺内分泌肿瘤表达ghrelin及GHS-R1A,患者血浆ghrelin及瘦素水平发生变化.

Objective To study the plasma and intratumoral levels of ghrelin, leptin and their relationship and clinical significance in patients with pancreatic endocrine tumor.Methods Preoperative plasma levels of ghrelin and leptin were detected by ELISA in 11 patients with pancreatic endocrine tumors and 28 normal controls.Expressions of ghrelin and its receptor GHS-R 1A were tested in 11 tumors and 27 paired control tissues by immunohistochemistry staining, and they were correlated with the clinicopathological characteristics.Results The plasma levels of ghrelin was （ 16.0 ± 5.0） pg/ml, which was significantly lower than that in normal controls [ （21.0 ± 2.0） pg/ml, P = 0.047 ].The plasma levels of leptin was （0.34 ±0.03 ） ng/ml, which was not significantly different with that in normal controls [ 0.38 ± 0.04） ng/ml ].There was positive association between plasma levels of leptin and ghrelin （P =0.015 ）, but was not associated with clinicopathological parameters.The plasma levels of leptin in control group was positively associated with BMI （P = 0.002）, but they were not associated in patients with tumor.The expression rate of ghrelin in tumor tissue was significantly lower than that in control group （64% vs 100%, P = 0.004 ）.But the expression rate of GHS-R I A was not significantly different between the two groups.The expression of ghrelin and GHS-R1A in tumor was not significantly associated with clinicopathological parameters.Conclusions The ghrelin and its receptor GHS-R 1A were extensively expressed in pancreatic endocrine tumors, and the serum levels of ghrelin and leptin was changed.