MCP-1在小型猪慢性阻塞性胰腺炎组织中的表达及其意义

Expression of MCP-1 in the pancreas of piglet with chronic obstructive pancreatitis and its significance

目的 检测慢性阻塞性胰腺炎小型猪血单核细胞趋化蛋白-1（MCP-1）含量及胰腺组织MCP-1蛋白表达,探讨其在胰腺纤维化中的作用.方法 采用不全结扎主胰管方法建立小型猪慢性阻塞性胰腺炎模型,术后4、6、8 周分批处死动物.观察胰腺病理变化;胶原（VG）染色评估胰腺纤维化程度;ELISA法检测血MCP-1浓度;免疫组化法检测胰腺组织MCP-1、α-SMA、PDGF、TGF-β1和NF-κB表达.结果 造模成功14头（58.3%）.术后4周起胰腺体尾部呈萎缩性改变,间质纤维组织增生,炎细胞浸润,第8周改变最明显.胰腺纤维化Ⅰ期5头（35.7%）,Ⅱ期4头（28.6%）,Ⅲ期5头（35.7%）.术后4、6、8周造模成功组血MCP-1含量分别为（102.44±36.25）pg/m1、（97.84±28.67）pg/ml、（94.32±28.42）pg/ml,显著高于对照组的（10.42±5.86）pg/ml、（8.58±4.86）pg/ml、（8.22±4.58）pg/ml（P值均＜0.01）.对照组胰腺无MCP-1蛋白表达,造模成功组胰腺组织内见MCP-1蛋白表达,且MCP-1表达与α-SMA、PDGF、TGF-β1和NF-κB表达呈正相关.结论 MCP-1在慢性阻塞性胰腺炎胰腺纤维化过程中起重要作用.

Objective To determine the serum concentration of MCP-1 and the expression of MCP-1 protein in the pancreas in the piglet with chronic obstructive pancreatitis and to explore the role of MCP-1 protein in pancreatic fibrosisits.Methods The piglet model of chronic obstructive pancreatitis was established by incomplete ligation of the pancreatic duct.The piglets were sacrificed at 4, 6, 8 weeks after induction.Pathological changes of pancreas were examined.Pancreatic fibrosis was assessed by VG staining.Serum MCP-1 concentrations were detected by ELISA method.MCP-1 and α-SMA, PDGF, TGF-β1 and NF-κB protein expression were detected by immunohistochemistry.Results The induction was successful in 14 piglets （ 58.3% ）.Mild atrophic changes, interstitial fibrosis, chronic inflammatory cell infiltration could be observed in the body and tail of pancreas from the 4th week in the experimental group.The most obvious changes occurred in the 8th week.Stage Ⅰ pancreatic fibrosis occurred in 5 piglets （35.7%）, stage Ⅱ in 4 piglets （28.6%）, stage Ⅲ in 5 rats （ 35.7% ）.Seurm MCP-1 at 4, 6, 8 weeks was （ 102.44 ± 36.25 ） pg/ml,（97.84 ± 28.67） pg/ml, （ 94.32 ± 28.42 ） pg/ml, respectively, and was significantly higher than that in control group [ （ 10.42 ±5.86） pg/ml, （8.58 ±4.86） pg/ml, （8.22 ±4.58） pg/ml, P 〈0.01 ].There was no MCP-1 protein expression in the control group;MCP-1 protein was detected in the successful induction group, and MCP-1 expression was positively correlated with expressions of the PDGF, TGF-β1, α-SMA and NF-κB.Conclusions MCP-1 may play an important role in the course of pancreatic fibrosis in chronic obstructive pancreatitis.