炭/炭复合骨植入材料研究

Study on Implant Material of Carbon/Carbon Composites

为评价自制炭/炭复合骨植入材料的生物相容性和力学性能,采用等温化学气相沉积法制备炭/炭复合材料并测试其力学性能,通过细胞毒性实验、微核实验、致敏实验以及植入实验评价其生物相容性。炭/炭复合骨植入材料力学测试显示压缩和拉伸性能良好;细胞毒性实验显示材料浸提液对L-929小鼠成纤维细胞的生长、增殖无明显影响;实验组小鼠骨髓微核率为2.3‰±0.7‰;致敏实验中实验组动物皮肤有轻微的红斑和水肿,按照Magnusson和Kligman分级为0.188±0.40;试样植入股骨后周围组织炎症反应轻微,12周组织学和扫描电镜观察显示,实验组材料组织相容性良好;以上生物学定量指标评价的实验组材料与阴性对照组相比,均无统计学差异(P>0.05)。综合评价结果表明,炭/炭复合材料具有良好的力学性能和生物相容性,有广泛的临床应用前景。

This study was aimed to evaluate the biocompatibility and mechanical property of carbon/carbon composites.At first,carbon/carbon composites were prepared by chemical vapor deposition,and the mechanical property of carbon/carbon composites was tested.The biocompatibility of carbon/carbon composites was evaluated by cytotoxicity test,sensitization test,micronucleus test and implantation test.Mechanical property test showed such carbon/carbon composites are of good compression property and tension property.Cytotoxicity test showed that the leaching liquor of samples has no effect on the growth and proliferation of L-929 cells.The medullary micronucleus frequency of mouse was 2.3‰±0.7‰ in experiment group.The sensitization test showed that the skin of the subjects of experiment group had slight erythema and edema,which was 0.188±0.40 according to Magnusson and Kligman classification.Implantation test revealed that there was slight inflammation around the tissue after the implantation of sample.At 12 weeks,scanning electron microscopy and histopathological exam indicated that the samples of experiment group were of good histocompatibility;and in comparison with control group,there was no significant differences（P0.05）.So these kinds of samples have good biocompatibility, mechanical property and prospects of clinical application.