利用探针跳跃式扫描离子电导显微镜研究活体神经细胞拓扑结构

Hopping Probe Scanning Ion Conductance Microscopy and Its Applications to Topological Imaging of Live Neuronal Cells

探针跳跃模式扫描离子电导显微镜(HPSICM)技术是在克服传统连续负反馈控制扫描模式仅能扫描表面平坦的生物样品的不足后,所发展起来的一种新型非接触式高分辨率扫描探针显微镜(SPM)技术。本文简要地介绍了该技术的工作原理,并运用其在体外培养条件下分别对活体SK-N-SH神经母细胞瘤细胞和经神经生长因子(Never growth factor,NGF)诱导分化的神经元样PC12细胞的三维形貌进行了高分辨率扫描成像,为在生理条件下研究神经细胞的表面微观结构及其动态变化提供了一种全新的显微观测手段。

As a new kind of non-contacting high-resolution scanning probe microscopy,hopping probe scanning ion conductance microscopy（HPSICM） overcomes the disadvantages of the continuous feedback-control scanning mode of the conventional scanning ion conductance microscopy（SICM）,which has been restricted to imaging relatively flat biological samples.The basic operation principles of HPSICM were briefly introduced in this paper.Then the high-resolution 3D morphological images of the two live neuronal cell lines,SK-N-SH cells and NGF-differentiated neurite PC12 cells,were respectively acquired in their physiological culture environment using HPSICM.It is demonstrated that HPSICM provides a powerful microscopy for in-depth studying the microstructures and their dynamic changes of live neuronal cells in real time.