摘要
目的 观察二硫化碳(CS2)对孕鼠胚胎和子宫组织中基质金属蛋白酶(MMP)-2、MMP-9表达水平的影响,探讨CS2致胚胎毒性作用机制.方法 昆明种小鼠随机分为对照组、卵泡发育期染毒组、胚胎植入早期染毒组、胚胎植入晚期染毒组,染毒组分别在卵泡发育期、胚胎植入早期、胚胎植入晚期腹腔注射631.4 mg/kg CS2,1次/d,连续2 d.孕第9天检查胚胎植入和发育情况,应用明胶酶谱法检测胚胎和子宫组织中MMP-2、MMP-9表达水平.结果 与对照组(30.700±5.599)比较,植入晚期染毒组胚胎植入数(16.000±12.166)明显减少,差异有统计学意义(P<0.05).染毒后胚胎组织中MMP-2、MMP-9表达均降低,其中植入晚期染毒组MMP-2、MMP-9分别为0.6837±0.0929、0.7309±0.0822,卵泡发育期染毒组分别为0.6222±0.0997、0.7520±0.1068,与对照组(分别为1.0000±0.0710、1.0000±0.0413)比较,差异均有统计学意义(P<0.01);植入晚期染毒组孕鼠子宫组织中MMP-2(1.3153±0.3032)、MMP-9(5.0210±4.0307)表达水平较对照组(分别为1.0000±0.1771、1.0000±0.0996)明显升高,差异有统计学意义(P<0.01).结论 胚胎植入晚期暴露CS2后胚胎毒作用明显,胚胎和子宫组织中MMP-2、MMP-9表达水平异常可能是CS2致胚胎毒性的重要机制之一.
Objective To observe the effect of carbon disulfide(CS2)on the expression of matrix metalloproteinase(MMP)-2, MMP-9 in mouse embryo and utems tissues and to explore the mechanism of embryo toxicity induced by CS2.Methods At the phases of follicular development and embryonic implantation which was subdivided into early-implantation phase and late-implantation phase, mice were intraperitoneally exposed to CS2(the dosage was 631.4 mg/kg, and the volume was 0.1ml/10 g body weight)for 2 consecutive days.All indicators were got at the ninth day in gestation, and the expression of MMP-2 and MMP-9 in embryo and uterus tissues was analyzed by gelatin zymography.Results The number of implanted embryos significantly decreased after exposure at late-implantation phase(16.000±12.166)compared with those of the control (30.700±5.599, P<0.05).Expression of MMP-2 and MMP-9 in embryos declined obviously at the three reproductive phases(P<0.01), and the levels of MMP-2 and MMP-9 expression in embryos at the phases of late-implantation phase(0.6837 ±0.0929,0.7309±0.0822)and follicular development(0.6222 ±0.0997,0.7520±0.1068)were much lower than those of the control(1.0000±0.0710, 1.0000±0.0413,P<0.01).Expression of MMP-2 and MMP-9 in uterus significantly increased at the phase of late-implantation(1.3153 ±0.3032,5.0210±4.0307)compared with those of the control(1.0000±0.177 1, 1.0000±0.0996, P<0.0 1).Conclusion Embryo toxicity of CS2 is more obvious at the phase of late-implantation.Exposure to CS2 disturbs expression of MMP-2 and MMP-9 in embryo and uterus tissues, which might be one of the important factors contributed to embryo toxicity induced by CS2.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2010年第12期881-884,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金项目(30671745)
山东省自然科学基金项目(Y2006C39)
关键词
二硫化碳
基质金属蛋白酶
基因表达调控
发育期
Carbon disulfide
Matrix metalloproteinase
Gene expression regulation, developmental