心肺复苏后大鼠脑红蛋白变化及氯化血红素的作用

Effects of hemin on neuroglobin expression after cardiopulmonary resuscitation in rats

目的 观察心肺复苏（CPR）后大鼠脑皮质脑红蛋白（NGB）表达、神经功能评分（NDS）、大脑皮质病理的变化及氯化血红素（Hemin）干预的影响.方法 成年雄性SD大鼠120只,随机（随机数字法）分为3组（n=40）：对照组（A）、复苏组（B）、药物组（C）,建立窒息致大鼠心搏骤停CPR模型,观测大鼠自主循环恢复（ROSC）后各时间点（0.5 h,3 h,6 h,12 h,24 h）NGB表达、NDS及大脑皮质病理改变.实验数据作单因素方差分析,组间比较用Tukey检验.结果 与A组同时间点比较,B组ROSC后12 h,24 h NGB表达显著升高（P＜0.05或P＜0.01）,ROSC后各时间点NDS显著降低（P＜0.01）、脑组织形态学明显异常;C组ROSC后6 h,12 h,24 h NGB表达显著升高（P＜0.05或P＜0.01）,ROSC后3 h,6 h,12 h NDS显著降低（P＜0.01）.与B组同时间点比较,C组ROSC后12 h,24 hNGB表达和NDS均显著升高（P＜0.01）,ROSC后脑组织形态学异常明显减轻.结论 大鼠CPR后大脑皮质区NGB表达增加、NDS降低、脑组织形态学明显异常,Hemin能增加大脑皮层NGB表达、提高NDS、减轻皮层病理损伤,具有一定的脑保护作用.

Objective To study the effect of Hemin on the level of neuroglobin （NGB） in cerebral cortex,neurodeficit score （NDS） and pathological changes in cerebral cortex after cardiopulmonary resuscitation （CPR） in rats. Method A total of 120 male Sprague Dawley（SD） rats were divided randomly into control group（A）, CPR group（B） and Hemin group（C）. The animal model of cardiac arrest （CA） induced by asphyxia was established and CPR was performed. The NGB level in cerebral cortex, NDS and pathological changes in cerebral cortex were examined 0.5 h,3 h,6 h, 12 h, 24 h after restoration of spontaneous circulation （ROSC） in each group. Experimental data were analyzed by using one-factor analysis of variance and Tukey test. Results In comparison with group A, the levels of NGB were significantly higher 12 h,and 24 h after ROSC （P 〈0.05 and P 〈0.01）, the values of NDS were significantly lower at each interval after ROSC （ P 〈 0.01 ） ,and the pathological changes were more severe at each interval after ROSC in group B. In comparison with group A, the levels of NGB were significantly higher 6 h, 12 h and 24 h after ROSC （ P 〈 0.05 and P 〈 0.01 ）, and the values of NDS were significantly lower 3 h, 6 h, 12 h after ROSC （ P 〈 0.01） in group C. In comparison with group B, the levels of NGB were significantly higher 12 h and 24 h after ROSC, the values of NDS were significantly higher 12 h and 24 h after ROSC, and the pathological changes were less in group C. Conclusions The NGB level increased in cerebral cortex, the NDS level decreased and severe pathological changes occurred in rats after CPR. The hemin treatment up-regulated the level of NGB, improved the NDS, mitigated pathological changes, alleviating the cerebral injury after CPR.