乌司他丁对百草枯中毒大鼠肺脏病理变化的影响

Protective effect of Ulinastatin on lung pathological changes in paraquat intoxication rats

目的 探讨乌司他丁对百草枯中毒大鼠肺脏病理变化的影响.方法 SD大鼠60只随机（随机数字法）分为正常对照组（A组）、百草枯染毒组（B组）和乌司他丁治疗组（C组）,各20只.A组给予生理盐水1 mL一次性灌胃;B组给予PQ液40 mg/kg（稀释至1 mL）灌胃染毒,并每日给予1 mL生理盐水腹腔注射;C组给予PQ液40mg/kg（稀释至1 mL）灌胃染毒,给予UTI 2万U/kg,2次/d腹腔注射.在给药后7,14,21,28 d处死大鼠,用苏木素-伊红（HE）、Masson染色评价肺组织病理学变化的表达.数据采用SPSS 10.0统计软件处理,等级资料采用秩和检验,有统计学意义后进行两两比较（Bonferroni法）.结果 百草枯染毒后大鼠肺泡炎、肺纤维化程度积分明显增高,乌司他丁治疗组肺组织水肿、出血、炎性细胞浸润及纤维聚集程度减轻.结论 乌司他丁能明显抑制百草枯中毒大鼠肺炎症及纤维化程度.

Objective To evaluate the protective effect of Ulinastatin on pathological changes of lung induced by paraquat （PQ） intoxication in rats. Method Sixty female SD rats were randomly and equally divided into three groups （ n = 20 in each group）. On the experimental day, the rats of group A （control group） received 1 mL of saline intragastrically; the rats of group B （PQ, group） received 1 mL of PQ intragastrically （40 mg/kg diluted in saline） and then treated with saline injected intraperitoneally once a day, and the rats of group Group C （Ulinastatin treatment group） received 1 mL of PQ intragastrically （40 mg/kg,diluted in saline） and then treated with 1 mL of Ulinastatin （ 120 000 IU/kg） intraperitoneally twice a day. The rats （ n = 5 in each subgroup） were sacrificed on the 7th, 14th, 21st, and 28th day for observing the histopathological changes of the lung stained with hematoxylin-eosin stain （HE） and Masson＇s trichrome stain. Data were semi-quantitatively analyzed by SPSS10.0 software. Hierarchical data were analyzed by rank and inspection. The data that have significance analyzed by comparison test （Bonferroni methods）. Results Paraquat intoxication resulted in significant alveolitis and lung fibrosis. Ulinastatin treatment alleviated lung edema, hemorrhage, inflammatory cell infiltration, and fiber aggregation. Conclusions These data demonstrated the Ulinastatin exerted protective effects against PQ-induced lung injury. Ulinastatin could to be used in clinical management for PQ intoxication.