CR16在特发性无精子症患者睾丸组织中的表达

Expression of CR16 in the testis of patients with idiopathic azoospermia

目的:研究糖皮质激素区域表达基因16(corticosteroids and regional expression 16,CR16)在特发性无精子症患者睾丸组织中的表达,探讨CR16在精子发生中的作用。方法:采用免疫组化法和逆转录-聚合酶链反应(RT-PCR)检测CR16蛋白和CR16 mRNA在48例特发性无精子症患者(病例组)和10例正常生育男性(正常组)睾丸组织中的表达情况。结果:CR16蛋白表达于睾丸生精小管上皮,主要分布在支持细胞与生精细胞连接区,在特发性无精子症患者睾丸组织中的表达显著弱于正常生育男性睾丸组织中的表达。CR16 mRNA在病例组睾丸组织中的表达水平显著低于正常组。结论:特发性无精子症患者睾丸组织中CR16表达水平显著降低,这可能与无精子症相关。

Objective：To investigate the expression of corticosteroids and regional expression 16（CR16） in the testis of patients with idiopathic azoospermia and the role of CR16 in spermatogenesis.Methods：Immunohistochemistry and RT-PCR were used to detect the expression levels of the CR16 protein and mRNA in the testes of 48 patients with idiopathic azoospermia and 10 healthy men.Results：Immunohistochemistry showed that the CR16 protein expressed in the Sertoli cells and spermatids-binding region in the epithelium of seminiferous tubules.The level of the CR16 protein was markedly lower in the idiopathic azoospermia patients than in the healthy men,and RT-PCR also showed a significantly decreased level of CR16 mRNA in the testis of the patients.Conclusion：The expressions of the CR16 protein and mRNA decrease markedly in the testis of patients with idiopathic azoospermia,indicating a correlation with the pathogenesis of azoospermia.