血清促血管生成素在肝癌中的表达及影响

The expression of serous angiopoietin and its affeoh in hepatoceHular carcinoma

目的探讨促血管生成素-2（angiogehin-2，Ang-2）对肝细胞肝癌（Hepatocellularcarcinoma，HCC）诊断的临床意义，以期为临床提供有意义的诊断依据。方法采用酶联免疫吸附试验测定30例HCC患者、10例肝硬化患者和10例健康者血清Ang-2水平，同时监测AFP、CEA、CAl99、FERR等肝癌相关指标进行对比。结果HCC组血清Ang-2水平（1892．54±482．82）μg／L和肝硬化组Ang-2水平（1244．21±14．65）μg／L均显著高于健康对照组（943．01±12．52）pg／L（P〈0．01，P〈0．05），此外HCC组血清Ang-2水平亦显著高于肝硬化组（P〈0．01）。HCC患者血清AFP（4397．61±613．82）μg／L、CA199（64．88±10．71）U／mL、FERR（401．53±51．32）μg／L、CEA（4．69±1．00）μg／L水平显著高于健康对照组（P〈0．01）；其中合并肝硬化组血清Ang-2（2045．41±155．31）μg／L、AFP（5097．17±2830．22）μg／L、CAl99（67．53±9．35）U／mL、FERR（462．87±56．79）μg／L、CEA（5．41±2．25）μg／L水平均高于未发生肝硬化组，差别有统计学意义（P〈0．05，P〈0．01）。结论肝癌患者血清Ang-2增高与HCC的发生、发展有关，血清中Ang-2水平检测可能为HCC临床诊断提供依据。

Objective To investigate the clinical significance of serous angiopoietin - 2 （ Ang - 2 ） in hepatocelhdar carcinoma（HCC） and the feasibility of using it as a diagnostic indicator. Method The serous Ang -2 level in 30 hepatocellular carcinoma patients, 10 cirrhosis patients as well as 10 health controls was detected with enzyme linked immunosorbent assay（ELISA）. Result The Ang -2 level of cirrhosis group （1 244.21 ± 14.65） μg/L was higher than that of control group （943.01 ± 12.52 ）μg/L, while the level of HCC group （ 1 892.54 ± 482.82 ） μg/L was higher than that of both cirrhosis group and control group ; HCC group had higher level of AFP（4 397.61 ± 613.82 ） μg/L, CA199 （64.88 ± 10.71 ） U/mL, FERR （401.53 ± 51.32）μg/L and CEA（4.69 ± 1. 00）μg/L than that of control group（ P 〈 0.05 ） ;the patients of HCC group complicated with cirrhosis also had higher level of Ang -2 （2 045.41 ± 155.31 ）μg/L, AFP （5 097.17± 2 830.22 ） μg/L, CA199 （ 67.53 ± 9.35 ） U/mL, FERR （462.87 ± 56.79 ） μg/L and CEA （ 5.41 ± 2.25 ） μg/L than those without cirrhosis （ P 〈 0.05 ）. Conclusion The elevated Ang - 2 is relevant to the pathogenesis and advancement of HCC, while serous Ang -2 is a promising candidate tumol： marker for the diagnosis of HCC.