摘要
背景与目的:Survivin在肿瘤的发生、发展中起重要作用。近年来,Survivin在胃癌研究领域备受关注。本研究探讨Survivin mRNA在胃癌外周血中的表达及其临床意义。方法:以GAPDH作内参照,应用实时定量RT-PCR技术检测胃炎及化疗前胃癌各50例外周血中Survivin mRNA表达水平。结果:正常对照组(胃炎患者)外周血中未检测到Survivin mRNA表达。胃癌组患者Survivin表达检测率为86%(43例),其中28例出现转移的患者阳性率为100%,22例未出现转移者为68.2%(P<0.05)。Survivin mRNA的表达与年龄、性别及淋巴结转移均无相关性(P>0.05)。回归分析发现,Survivin mRNA的表达水平是影响胃癌预后的独立预测因素。结论:外周血Survivin mRNA可作为临床评估胃癌患者预后的客观指标之一。
Background and purpose：Recently,pivotal studies have extensively investigated the role of the survivin gene in gastric cancers.This study aimed to investigate the clinical significance of the expression level of survivin mRNA in the peripheral blood of gastric cancers.Methods：The expression levels of survivin mRNA in the peripheral blood of gastric cancer and gastritis were detected by Real-time quantitative reverse transcription polymerase chain reaction（RT-PCR）,while GAPDH was used as an internal reference gene.Results：Specimen from gastritis patients and gastric cancer patients indicated expression rates from survivin mRNA as 0%,and 86%.The expression of survivin mRNA showed no correlation with age,gender or lymph node metastasis（P0.05）,but was significant different in the TNM stage（P0.05）.The cox stepwise proportional hazards analysis showed that the expression levels of survivin mRNA remained significantly independent during prognosis.Conclusion：The expression levels of survivin mRNA was overexpressed in the peripheral blood of gastric cancer.These expression levels may also be an important marker for prognosis for gastric cancer patients.
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2010年第12期903-906,共4页
China Oncology
基金
江苏省常州市武进区科技局资助项目(No:WS2009007)
