贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法研究

A Tricine-SDS-PAGE Electrophoresis Method for Determination of Kinase Components in Guizhou Douchi

目的:建立一种能够稳定检测贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法。方法:采用Tricine-SDS-PAGE电泳方法,分离胶浓度为12%,交联度为3%,以blue-silver染色法进行染色,对贵州豆豉激酶组分进行分析。结果:贵州豆豉激酶组分经Tricine-SDS-PAGE电泳后,在蛋白Marker在20.1～43 kDa之间,出现5条较清晰的条带。结论:Tricine-SDS-PAGE电泳方法稳定可靠,能够用于贵州豆豉激酶组分的检测。

Objective： To establish a tricine-SDS-PAGE method for stable determination of kinase components in Guizhou douchi.Method： Tricine-SDS-PAGE was used,of which the gel concentration and cross-linking density were 12% and 3%,respectively.After electrophoresis,the gel was stained with ＂blue-silver＂ method.Results： Five bands between 20.14 kd and 43 kd were found on the electrophoretogram.Conclusion： Tricine-SDS-PAGE could detect the components of Guizhou douchi steadily and reliably.