部分析因设计优化双酶法水解橡子淀粉的工艺研究

Study on Double Enzymatic Hydrolysis of Acorn Starch Optimized by Factorial Design

以部分析因设计考察了不同因素对双酶法水解橡子淀粉的影响。通过分析,得出主要影响因素为淀粉酶用量、液化时间、糖化时间、糖化酶用量。并对其4个主要因素进行正交试验优化,得出最佳的工艺条件为:淀粉酶用量50 U/g,液化时间2 h,糖化时间2 h,糖化酶用量600 U/g。此条件下的橡子淀粉水解度(DE)值为58.15%。

Effects of double enzymatic hydrolysis of acorn starch were studied by factorial design.The main factors,namely amylase dosage,liquefaction time,glucoamylase dosage and saccharification time were optimized by orthogonal tests.Through analysis,the optimal conditions were obtained as follows： amylase dosage 50 U/g,liquefaction time 2 h,saccharification time 2 h,and glucoamylase dosage 600 U/g.Under these conditions the dextrose equivalent value（DE） of the acorn liquefied solution was 58.15 %.