超声波辅助萃取法提取乌药叶黄酮类化合物的工艺研究

Ultrasound-assisted Extraction of Flavonoids from Leaves of Lindera aggregata(Sims.) Kosterm

以乌药叶为原料,用均匀设计试验法优化了乌药叶总黄酮(FL)的超声波提取工艺,考察了乙醇体积分数、液固比、超声波功率和超声波作用时间4个因素对乌药叶总黄酮提取率的影响,确立了乌药叶总黄酮的优化超声波提取工艺条件为:5 g乌药叶粉,超声波作用时间18 min,超声波功率250 W,乙醇体积分数40%,液固比10∶1(mL∶g),测得乌药叶总黄酮得率为2.13%。对超声波提取进行了数学模拟,模拟值与试验结果吻合良好。

Ultrasound-assisted extraction of flavonoids in leaves of Lindera aggregata（Sims.）Kosterm（FL） was optimized by an uniform design experiment.The effects of ethanol volume fraction,ratio of liquid to solid,ultrasound power and ultrasound treatment time on the extraction yield of FL were investigated.The optimized ultrasound-assisted extraction was determined as follows：5 g leaves of FL,ultrasound treatment time 18 min,ultrasound power 250 W,ethanol volume fraction 40 %,ratio of liquid to solid 10∶1（mL∶g）.Under these conditions,the extraction yield of FL reached 2.13 %.Mathematical simulation values based on ultrasound-assisted extraction was consistent with experimental values.