正常人角膜内皮细胞的增龄性变化

Age-related changes of the corneal endothelial cells in healthy subjects

目的了解正常人角膜内皮细胞的增龄性改变。方法采用日本Topcon角膜内皮计,对180例(343只眼)6～94岁不同年龄正常人角膜进行5个方位检查,分析不同年龄个体角膜内皮细胞密度、平均面积、标准偏差和面积变异系数、六角形细胞所占比例以及中央角膜厚度等结构参数的状况。结果受检者平均年龄(40.77±22.02)岁(6～94岁),平均角膜内皮细胞数目为(128.08±17.24)个,平均密度为(2894.64±372.42)个/mm2,平均六角形细胞所占比例为(55.40±7.90)%,上述3个参数随年龄增长而下降,尤以30岁以后变化更明显。平均角膜内皮细胞面积、平均变异系数及细胞面积标准偏差分别为(353.27±52.56)μm2、(36.25±6.74)%和(128.48±32.46)μm2,呈现随年龄增长而数值增大的趋势;平均角膜厚度为(0.55±0.03)mm,各年龄组间无显著性差异。结论正常人角膜内皮细胞密度和形态随年龄变化而改变,总体呈现随着年龄的增长角膜内皮细胞数目和密度降低、细胞面积增大、变异系数增大、六角形细胞比例减少的趋势。

Objective To observe the changes of corneal endothelial cells in different age ranges.Methods 343 eyes of 180 healthy subjects aged from 6 to 94 years were evaluated using Topcon corneal specular microscope and the changes of cells density,averaged size,standard deviation,coefficient of variation and the proportion of hexagonal cells,as well as central corneal thickness and other parameters of corneal endothelial cells were analyzed in different age ranges.Results Mean ages of healthy subjects were （40.77±22.023） years old. The averaged corneal endothelial cell numbers were 128.08±17.24,averaged endothelial cell density was （2894.64±372.42）/mm2,and the proportion of hexagon cell was （55.40±7.90）%,which showed decreasing tendency with age,especially after 30 years old. The averaged cell area was （353.27±52.56）μm2,averaged coefficient variation was （36.25±6.74）,and the standard deviation was 128.48±32.46 respectively,which showed increasing tendency with age. The averaged central corneal thickness was （0.55±0.03）mm,there is no significantly different among all groups.Conclusion The numbers,density and morphology of corneal endothelial cells are changing with age in various age-groups of healthy human.