孤立性肥大细胞瘤1例的电镜观察及国内文献综述

Investigation on a Case of Solitary Mastocytoma by Elcetronic Microscopy and Chinese Literature Review

目的:总结肥大细胞瘤的临床病理特点。方法:1例婴儿孤立性肥大细胞瘤的皮损活检标本行组织病理、姬姆萨染色和透射电镜检查。结果:皮损组织病理检查显示真皮内大量肥大细胞弥漫性浸润,姬姆萨染色显示胞质中存在紫红色异染颗粒。电镜检查显示浸润细胞主要为形态正常的肥大细胞,也有一些嗜酸粒细胞和巨噬细胞。高密度的夏科-莱登结晶游离于间质中,并与损伤的核染色质、细胞膜碎片、肥大细胞释放的颗粒相混合。结论:肥大细胞瘤多在出生时发病,确诊有赖于组织病理检查。

Objective：To summarize the clinicopathologic features of mastocytoma. Methods：The biopsy specimen of an infant with solitary mastocytoma was detected by HE,Giemsa stain and transmission electron microscopy（TEM）.Results：HE stain showed there were numerous of mast cells in the entire dermis,and Giemsa stain demonstrated the presence of purple-red metachromatic granules within cytoplasm.TEM exhibited that the cellular infiltrate was mainly composed of mast cells with normal appearance,but some eosinophils and macrophages were also noticed.Electron dense Charcot-Leyden crystals were free in stromal tissue,and admixed with damaged nuclear chromatin,membrane debris,and granules released from mastocytes.Conclusion：Mastocytoma mostly onset at birth,and its definite diagnosis depends on the histopathology.