丹红注射液促肝癌细胞凋亡及NDRG2表达的初步研究

Danhong injection enhances apoptosis index and NDRG2 expression in hepatocellular carcinoma cells

目的观察丹红注射液对肝癌细胞的凋亡及抑癌基因NDRG2表达的影响。方法将0μl/ml、0.5μl/ml、1μl/ml、2μl/ml的丹红注射液作用人肝癌细胞系HepG2,24h后光镜观察细胞形态改变;流式细胞仪检测细胞凋亡指数变化,选择2μl/ml作用后0h、6h、12h、24h后流式细胞仪检测细胞凋亡指数变化;Real time PCR检测细胞NDRG2基因mRNA表达水平。结果丹红注射液作用HepG2细胞24h后,2μl/ml组较其他浓度组光镜下呈现较明显细胞形态改变,流式细胞仪检测细胞凋亡指数增加,并且随作用时间的延长,细胞凋亡指数也增加。Real time PCR检测显示,2μl/ml组随着作用时间的延长,细胞凋亡指数的增加;细胞的抑癌基因NDRG2mRNA表达水平也随之增高。结论丹红注射液可诱导肝癌细胞系HepG2的抑癌基因NDRG2表达,促进细胞凋亡,且具有一定的剂量、时间依赖性。

Objective To explore the changes of apoptosis index and NDRG2 expression in hepatocellular carcinoma cells after cultured with Danhong injection. Methods The hepatocellular carcinoma cell line HepG2 was given different concentrations of 0 μl/ml,0.5 μl/ml,1 μl/ml,2 μl/ml Danhong injection,respectively.The morphology was observed under light microscope after 24 h.The apoptosis index was detected by flow cytometry and NDRG2 gene expression was determined by real time-PCR at 0 h,6 h,12 h,24 h after cultured with 2 μl/ml Danhong injection. Results The HepG2 cells showed a obvious morphological change of apoptosis and a great number of dead cells under light microscope after cultured with 2 μl/ml Danhong injection for 24 h,and the apoptosis index increased in a time-dependent manner.The NDRG2 mRNA expression was significantly higher at 24 h than that at 0h after the HepG2 cells was given 2 μl/ml Danhong injection. Conclusion Danhong injection could induce the increase of NDRG2 expression and promote the cell apoptosis in hepatocellular carcinoma in a dose-and time-dependent manner.