脂质体猪苓多糖抗肝转移癌作用的研究

Inhibition of experimental liver tumor growth in mice by liposomal PUPS

目的 观察脂质体猪苓多糖抗Ｂ１６ 黑色素瘤肝转移癌的作用。方法 将制备的Ｂ１６ 黑色素瘤单细胞悬液注入同系小鼠脾内制备肝转移癌小鼠模型，用游离猪苓多糖（ 每次每只小鼠６０μｇ） 、空白脂质体和脂质体猪苓多糖（ 含猪苓多糖６０μｇ） 分别处理小鼠，１５ 天后观察小鼠肝表面的癌结节数目。结果 脂质体猪苓多糖能显著减少Ｂ１６黑色素瘤肝转移癌结节数目（ Ｐ＜ ０ ．０１） ，空白脂质体组和游离猪苓多糖组小鼠肝转移癌结节数目，与对照组比较无明显差异（ Ｐ＞０ ．０５） 。结论 脂质体猪苓多糖具有抑制Ｂ１６

Aim To investigate the inhibition of B 16 melanoma liver metastases growth in C 57 BL/6 mice by liposomal Polyprous umbellatus polysaccharide(PUPS) .Methods An mouse model of B 16 melanoma liver metastases was established by injection of single cell suspensions of tumor into the spleen.The mice were treated with free PUPS(60μg),MLV PBS and MLV PUPS(Containing PUPS 60μg) respectively.Results Treatment of mice with MLV PUPS significantly reduced the number of hepatic metastatic nodules in the mice,MLV PBS and free PUPS failed to affect the number of liver metastases.Conclusion Liposomal encapsulation of PUPS are effective in inhibiting the growth of liver metastases in mice.