慢性肝炎患者高压氧治疗前后肝组织中HBsAg、HBcAg检测初步探讨

General study on the changes of HBsAg、HBcAg in the liver tissues of patients with chronic hepatitis treated with hyperbaric oxygenation

目的 观察慢性肝炎（ＣＨ） 高压氧（ ＨＢＯ） 治疗前后患者的免疫功能、肝组织中ＨＢｓＡｇ 、ＨＢｃＡｇ 变化。方法：用纯氧单仓治疗（２－５ＭＰａ ，２ｈ／ｄ ，１０ｄ／ｃｙｃ ，６ｃｙｃ）３０ 例ＣＨ，治疗前后用美国ＢＥＣＭＡＮ 公司生产ＡｒｒａｙＴＭＰｒｏｔｅｉｎ Ｓｙｓｔｅｍ ３６０ 测全部患者静脉血白蛋白（ Ａ） 、γ球蛋白（γ） 、ｌｇＡ、ｌｇＧ、ｌｇＭ、和补体Ｃ３ 、Ｃ４ ；二次肝穿刺活检，ＡＢＣ 法检测肝组织中ＨＢｓＡｇ 、ＨＢｃＡｇ 。取算术均数行ｔ 检验。结果：ＨＢＯ 治疗后静脉血γ、ｌｇＧ、ｌｇＭ、明显降低，Ａ、Ｃ３ 明显升高，治疗前后差异显著（ Ｐ＜０－０５） ；肝组织中ＨＢｓＡｇ 、ＨＢｃＡｇ 变化不明显，治疗前后差异不显著（ Ｐ＞ ０－０５） 。结论：ＨＢＯ 治疗ＣＨ，可抑制患者的免疫功能，对ＨＢｓＡｇ 、ＨＢｃＡｇ

Aim To observe the changes of the HBsAg、HBcAg in the liver tissues and the immunity of patients with Chronic hepatitis(CH)treated with hyperbaric oxygenation(HBO).Methods 30 cases with CH were treated with the HBO( 2.5 Mpa,2h/d,10d/cyc,6cyc).Before and after the HBO treatment,using the Array Protein System(BECMAN:Array360)equipment,the albumin(A)、γ globulin(γ) IgG、IgM、IgM、and complement C3、C4 in venous blood were determined.The HBsAg and HBcAg in the liver tissue biopsy specimens were detected with immunohistochemistry(ABC).Results After the HBO treatment,the γ、IgG、IgM、IgA and C4 were reduced and the A、C3 were increased( P <0 05) The changes of the HBsAg、HBcAg in the liver tissue biopsy specimens were not statistically significant( P >0 05) Conclusion HBO has effects on reducing the immunity but not on resisting HBV for the paitents with chronic hepatitis.