miR-106b转基因小鼠的建立

Establishment of miR-106b Transgenic Mouse

目的:建立miR-106b转基因小鼠模型,探讨其在阿尔茨海默病(Alzheimer’s d isease,AD)发病中的作用。方法:构建miR-106b表达载体,显微注射法建立miR-106b转基因小鼠。PCR鉴定转基因小鼠的基因型,real time RT-PCR检测m iR-106b转基因小鼠脑组织中miR-106b的表达情况,W estern blot检测miR-106b转基因小鼠脑组织中TGFBR2蛋白的表达。结果:构建了高表达m iR-106b转基因小鼠;与对照相比,miR-106b转基因小鼠脑组织中TGFBR2蛋白的表达升高。结论:miR-106b转基因小鼠的建立为研究该microRNA在AD发病中的作用提供了工具。

Objective：To establish a miR-106b transgenic mouse and investigate its effect on the development of Alzheimer＇s disease（AD）.Methods：The expression vector of miR-106b was constructed.The transgenic mouse was produced by microinjection and genotype was detected by PCR.The expression levels of miR-106b were detected by real time RT-PCR.The protein levels of TGFBR2 were detected by Western blot.Results：Five founders of miR-106b transgenic mice were established and one high-level expression line was identified.Compared with the wild type mice,the expression of TGFBR2 was increased in miR-106b transgenic mice.Conclusion：miR-106b transgenic mouse has been established and it can be used to investigate the function of miR-106b on the development of Alzheimer＇s disease.