毕赤酵母发酵生产T4溶菌酶的中试研究

The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris

研究了T4溶菌酶的中试发酵放大生产。在200L发酵罐中毕赤酵母诱导表达T4溶菌酶蛋白,发酵液蛋白表达量达到1.69g/L,酶活达到192U/mg;制得冻干酶粉211.1g。经测定粗酶粉中T4溶菌酶的含量达到68.9%,酶活为182U/mg,总收率达到66%,成功建立了一条T4溶菌酶的中试发酵生产工艺线路。

T4 lysozyme pilot-scale fermentation was studied.T4 lysozyme was induced and expressed by Pichia pastoris in the 200L fermentor.The yield of target protein was 1.69g /L with 192U/mg enzyme activity in the broth.The enzyme protein was freeze-dried.The weight of enzyme powder was 211.1g.T4 lysozyme content was 68.9%,and activity was 182U/mg in enzyme powder.The total yield was 66%.The T4 lysozyme pilot-scale process line was established successfully.