期刊文献+

人牙周膜细胞不同生物学特性的表达 被引量:6

Expression of different biological characteristics of human periodontal ligament cells
下载PDF
导出
摘要 目的:观察人牙周膜细胞生物学特性表达。方法:采用细胞克隆技术,以细胞的牙骨质附着蛋白( C A P) 亲和力、碱性磷酸酶( A L P) 表达率和细胞的钙化能力为指标,对5 名患者的5 个正常前磨牙的牙周膜( P D L) 细胞进行了初步的鉴定。结果:在接种的669 个克隆细胞中,仅有43 .6 % 的克隆细胞具有持续增殖潜力。在这些生长克隆细胞中,非钙化细胞所占比例要多于钙化细胞,但这两类细胞中分别存在着 A L P 表达和 C A P 亲和力有着不同表现的细胞。结论:牙周膜中存在有不同表现型的细胞。提示牙周组织再生是一复杂的过程。这些细胞在牙周组织再生中起着什么作用,尚待进一步研究。 Aim: Expresions of biologieal characteristics of human periodontal liagment cells was observed. Methods: The cells derived from human periodontal ligament of 5 donors were classified based on CAP binding, ALP expression and mineralized-tissue formation using cell cloning technique. Six hundred and sixty nine clones were observed, but only 43.6% clones that developed into clonies were monitored. Results: the number of non-mineralizing-clone of growing clones was more than that of mineralizing-clone. These two type clones showed difference in CAP binding and ALP expression. Conclusions: This result indicated that there was different cell phenotype in growing clones of periodontal ligament cells. The behavior of the subtype cells and their role it played in periodontal regeneration should be further studied.
出处 《牙体牙髓牙周病学杂志》 CAS 1999年第3期191-194,共4页 Chinese Journal of Conservative Dentistry
基金 国家自然科学基金
关键词 牙周膜细胞 细胞生物学 表达 human periodontal ligament cells cell biology clone
  • 相关文献

同被引文献26

  • 1杜丽珍.根管治疗一次法术后反应的发生率及其相关因素[J].现代口腔医学杂志,2005,19(2):222-222. 被引量:50
  • 2李德懿.牙周病微生物学[M].天津:天津科技翻译出版公司,2004:119一123.
  • 3Orstavik D. Time-course and risk analyses of the developmenrand healing of chronic apical periodontitis in man[J]. Int Endod J, 1996,29(3) : 150-155.
  • 4Ito K,Nishikata J,Murai S Effect of Nd:YAG laser radiation on removal of root surface smear layer after root planing:SEM study[J] J Periodontol,1993,64:547-552.
  • 5Liu CM,Shyu YC,Pei SC,et al In vitro effect of laser irradiation on cementum-bound endotoxin isolated from periodontally diseased roots[J] J Periodontol,2002,73:1260.
  • 6Gaspirc B,Skaleric U Morphology,chemical structure and diffusion prossess of root surface after Er:YAG and Nd:YAG laser irradiation[J] J Clin Periodontol,2001,28:508-516.
  • 7赵福运.实用激光治疗学[M].北京:北京医科大学、中国协和医科大学联合出版社,1997.8-21.
  • 8Lin DG, Kenny DJ, Barrett EJ, et al. Storage conditions of avulsed teeth affect the phenotype of cultured human periodontal ligament cells [J]. J Periodont Res, 2000, 35:42-50
  • 9Parkar MH, Kuru L, Giouzeli M, et al. Expression of growth-factor receptors in normal and regenerating human periodontal cells [J]. Arch Oral Biol, 2001, 46(3):275-284
  • 10Matsuda N, Kumar NM, Ramakrishnan PR, et al. Evidence for up-regulation of epidermal growth-factor receptors on rat periodontal ligament fibroblastic cells associated with stabilization of phenotype in vitro [J]. Arch Oral Biol, 1993, 38(7):559-569

引证文献6

二级引证文献42

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部