VEGF与MMP-9在原发性肝癌中的表达及意义

Expression and significance of VEGF and MMP-9 in patients with primary hepatocelluar carcinoma

目的探讨原发性肝癌患者血管内皮生长因子(VEGF)和基质金属蛋白酶-9(MMP-9)的表达水平及其意义。方法采用酶联免疫吸附试验(ELISA)检测30例原发性肝癌患者和20例健康对照者外周血血清中VEGF和MMP-9的水平,应用半定量逆转录-聚合酶链反应方法检测30例原发性肝癌和20例正常肝组织中的VEGFmRNA及MMP-9mRNA的表达。结果肝癌患者外周血血清VEGF和MMP-9两种蛋白水平明显高于健康对照组;肝癌组织VEGFmRNA和MMP-9mRNA的表达明显高于正常肝组织。结论 VEGF及MMP-9在肝癌的发生、发展中可能起着重要作用,检测血清VEGF、MMP-9及肝癌组织中VEGFmRNA及MMP-9mRNA水平可能对肝癌诊断具有一定临床指导意义。

Objective To investigate the expression and significance of vascular endothelial growth factor （VEGF） and matrix metalloproteinase-9 （MMP-9） in patients with primary hepatocellular carcinoma （PHC）. Methods Serum levels of VEGF and MMP-9 of 30 patients with PHC and 20 cases of normal controls （NCs） were measured by enzyme linked immunosorbent assay （ELISA）. mRNA levels of VEGF and MMP-9 in hepatic tissues from all subjects were detected by semi quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results ： The serum levels of VEGF and MMP 9 in the patients with PHC were significantly higher than those in NCs. The mRNA levels of VEGF and MMP-9 in hepatic tissues from patients with PHC were sig- nificantly higher than those from NCs. Conclusion VEGF and MMP 9 may play important roles in the onset and development of PHC. The levels of VEGF and MMP 9 may have very important clinical guiding significance in the diagnosis of PHC .