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重组PrP102-242在COS-1中的表达及表达产物的检测

Detection and expression of recombinant protein PrP102-242 in COS-1 cells
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摘要 目的重组的PrP102-242在COS-1细胞中的稳定表达以及表达产物的检测。方法重组表达载体pCI-PrP102-242转染COS-1细胞后,经不同浓度的G418筛选后获得稳定的细胞系,然后用间接免疫荧光、间接ELISA和Western blot检测目的蛋白的表达。结果间接免疫荧光、间接ELISA和Western blot检测到目的蛋白在COS-1细胞中的表达。结论目的蛋白PrP102-242在COS-1细胞中的成功表达为下一步研究PrPc的结构和功能奠定了基础。 To detect the expression of product of recombinant protein PrP102-242 in COS-1 cells,purified recombinant protein of PrP102-242 were transfected into COS-1 cells,to obtain stable cell lines using different concentration of G418.After that,the expression of recombinant protein PrP102-242 in COS-1 were detected by using indirect immunity fluorescence assay（in-IFA）,indirect enzyme linked immunosorbent assay（in-ELISA）and Western blot assay.The result showed that expression of pCIp264 in COS-1 were detected by using in-ELISA,IFA and Western blot.
作者 丁耀忠 刘永生 陈浩泰 刘文倩 张杰
机构地区 中国农业科学院兰州兽医研究所
出处 《中国人兽共患病学报》 CAS CSCD 北大核心 2010年第12期1097-1100,共4页 Chinese Journal of Zoonoses
基金 国家自然科学基金(No.30671563)资助
关键词 朊蛋白 COS-1细胞 间接免疫荧光检测技术 prion protein（PrP） COS-1 indirect immunofluorescence
分类号 Q786 [生物学—分子生物学]
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参考文献10

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中国人兽共患病学报
2010年 第12期

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