鞘内注射和侧脑室注射七氟烷对小鼠抓力的影响研究

Effect of Sevoflurane on the Grip Strength of Mice by Intrathecal and Intracerebroventricular Injection

目的:研究七氟烷(Sev)产生肌松作用的主要部位。方法:将64只小鼠按基础抓力、体重,用分层随机区组设计均分为鞘内(it)注射组和侧脑室(icv)注射组,每组再各分为人工脑脊液(aCSF)组(0.25μL·g-1)、Sev1组(0.25mg·g-1)、Sev2组(0.31mg·g-1)、Sev3组(0.39mg·g-1),每小组8只,给予相应药物,注射时间为10s,留针时间为15s,考察各组小鼠给药前和给药后5、10、15、20、25、30min的抓力变化。结果:与aCSF组比较,it和icv注射Sev各组小鼠抓力均减小,且注射Sev浓度越高小鼠抓力越小,其中Sev2组和Sev3组具有显著性差异(P<0.05或P<0.01);与icv组比较,it组中的Sev2组、Sev3组小鼠抓力均显著减小(P<0.01)。结论:it和icv注射Sev后均可产生剂量依赖性的肌松作用,但相同剂量下it注射后作用更强,提示脊髓可能是Sev产生肌松作用的主要部位。

OBJECTIVE： To study the main site of muscle-relaxation induced by sevoflurane （Sev）. METHODS： 64 mice were randomly divided into intrathecal injection （it） group and intracerebroventricular injection （icv） group. Then it group and icv group were redivided into aCSF group （0.25 μL·g-1）, Sev1 group （0.25 mg·g-1）, Sev2 group （0.31 mg·g-1）, Sev3 group （0.39 mg·g-1） with each group of 8 mice. The duration of injection was 10 s and acupuncture manipulation time lasted for 15 s. The grip strength of mice were recorded before administration and 5, 10, 15, 20, 25, 30 min after administration. RESULTS： Compared with aCSF group, the grip strength of mice in each group had reduced as the concentration of Sev increased, there was significant difference between Sev2 group and Sev3 group （P0.05 or P0.01）. Compared with icv groups, the grip strength of mice in Sev2 group and Sev3 group of it group had reduced （P0.01）. CONCLUSION： Intrathecal and intracerebroventricular injection of sevoflurane produce muscle relaxation effect in dose-dependent manner, especially in it group. It can be concluded that spinal cord is the main site of muscle-relaxation induced by sevoflurane.