HPLC法同时测定痤疮清软膏中甲硝唑与维生素B_6的含量

Simultaneous Determination of Metronidazole and Vitamin B6 in Cuochuangqing Ointment by HPLC

目的:建立同时测定痤疮清软膏中甲硝唑、维生素B6含量的方法。方法:采用反相高效液相色谱-切换波长法。色谱柱为DiamonsilTMC18,流动相为甲醇-水(10∶90),0～6min、6.1～11min时检测波长分别为291、277nm,柱温为30℃,进样量为10μL,流速为1.0mL·min-1。结果:甲硝唑、维生素B6进样量线性范围分别为7.796～38.98μg、0.272～1.360μg(r均为0.9997);甲硝唑、维生素B6的回收率分别为98.9%(RSD=1.1%)、97.0%(RSD=0.5%)。结论:所建立的方法操作简便、专属性高、重复性好,结果准确、可靠,可为痤疮清软膏质量控制提供依据。

OBJECTIVE： To establish a method for simultaneous determination of metronidazole and vitamin B6 in Cuochuang- qing ointment. METHODS： RP-HPLC wavelength switching method was adopted. DiamonsilTMC18 column was used with mobile phase consisted of methanol-water （10 ∶ 90）. The detection wavelength was 291 nm at 0～6 min and 277 nm at 6.1～11 min and column temperature was set at 30 ℃. The flow rate was 1.0 mL·min-1 and injection volume was 10 μL. RESULTS： The linear range of metronidazole was 7.796～38.98 μg and that of vitamin B6 was 0.272～1.360 μg （r=0.999 7）. Average recoveries were 98.9% （RSD=1.1%） and 97.0% （RSD=0.5%）, respectively. CONCLUSION： The method is simple, specific and reproducible, accurate, reliable and suitable for quality control of Cuochuangqing ointment.