中国拉祜族人HLA-DR4的快速DNA分型

FAST DNA GENOTYPING OF HLA DR4 IN LAHU CHINESE

应用扩增阻滞突变系统（ａｍ ｐｌｉｆｉｃａｔｉｏｎ ｒｅｆｒａｃｔｏｒｙ ｍ ｕｔａｔｉｏｎ ｓｙｓｔｅｍ ，ＡＲＭＳ）对１１０例云南拉祜族人进行ＨＬＡ－ＤＲ４基因分型，结果显示拉祜族人ＨＬＡ－ＤＲ４基因纯合子占４．５％ ，杂合子占１５．５％ ，非ＤＲ４占８０％ 。ＨＬＡ－ＤＲ４基因频率为０．１２２７，与我国已报道的北方、南方汉族和彝族ＨＬＡ－ＤＲ４频率基本一致，而与日本人、高加索人群存在显著差异。分型结果为人类学研究及ＨＬＡ 相关疾病研究提供了参考依据。

HLA DR4 genotypes of 110 unrelated healthy Lahu Chinese were screened by using ARMS.Among them,5 individuals were homozygous for DR4,17 were heterozygous for DR4,and the rest 88 were non DR4.The gene frequency (GF) of DR4 in Lahu Chinese was 0.1227.Comparisons of GF of DR4 in different populations showed there were similar HLA DR4 GFs in Chinese populations and significant differences between Chinese and Japanese or Caucasians.The study showed ARMS was a simple,rapid and reliable technique for HLA DR4 genotyping.The results obtained will also be useful to further researches on HLA related diseases and anthropology.