CD 59在sM AC诱导EL-4细胞增殖效应中的作用

POLE OF CD59 IN sMAC INDUCED EL 4 CELL PROLIFERATION

以表达人ＣＤ５９ｃＤＮＡ 及ＣＤ５９－ＴＭｃＤＮＡ 的小鼠胸腺瘤（ＥＬ－４）细胞为研究对象，探讨了ＣＤ５９在ｓＭＡＣ诱导ＥＬ－４细胞增殖效应中的作用。结果发现：１）用抗体交联ＣＤ５９分子对ＣＤ５９／ＥＬ－４细胞的增殖有促进效应，而对ＣＤ５９－ＴＭ／ＥＬ－４细胞的增殖无明显影响（Ｐ＜ ０．０１）；２）ｓＭＡＣ对ＣＤ５９／ＥＬ－４细胞的增殖有促进作用，而对ＥＬ－４及ＣＤ５９－ＴＭ／ＥＬ－４细胞则均未出现上述现象（Ｐ＜ ０．０１）；３）阻断信号传导的蛋白酪氨酸激酶途径对上述ＣＤ５９及ｓＭＡＣ介导的ＣＤ５９／ＥＬ－４细胞的增殖有不同的抑制效应。结果提示：ＣＤ５８参与了ｓＭＡＣ诱导ＥＬ－４细胞增殖的效应过程。

The mechanism of sMAC induced T cell activation and proliferation is still unknown.Murine thymoma cell line EL 4,which express either natural CD59 or transmembrane form of CD59 were used to investigate the role of CD59 in this process.Results showed that 1)cell proliferation induced by cross linking CD59 with antibody can only be found in CD59/EL 4 cell( P <0.01).2)CD59/EL 4 cell can response to sMAC stimulation,resul ting in proliferation,while EL 4 and CD59 TM/EL 4 dont have such response( P <0.01).3)CD59 and sMAC induced C59/EL 4 cell proliferation response differently to PTK inhibition.