骨髓基质干细胞在等离子体处理PEGDA基凝胶表面的黏附和增殖

Adhesion and Proliferation of BMSCs on the Surface of PEGDA-based Hydrogel by Low-temperature Plasma

通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通过X射线光电子能谱、接触角和扫描电镜进行表征.研究结果表明,材料表面经氩等离子体处理后,其亲水性得到较大的改善,表面自由能由45.9 m J/m2增加到70.3 m J/m2;体外实验结果证明,BMSc在等离子体处理后材料表面培养24 h后出现明显细胞核,168 h细胞融合成片,通过等离子体处理方法有利于细胞在水凝胶材料表面的黏附和增殖.

Argon plasma treatment was used to improve the biocompatibility of the surface of photo-polymerization poly（ethylence glycol） diacrylate（PEGDA）/2-hydroxyethyl methacrylate（HEMA） hydrogel.The adhesion and proliferation of bone marrow mesenchymal stem cells（BMSCs） on the surface of the films was investigated.The surface structures of the PEGDA-based films before and after treated by Ar plasma were characte-{rized} by scanning electron microscope（SEM）,X-ray photoelectron spectroscopy（XPS） and contact angle.The results confirm that the surface energy of PEGDA/HEMA hydrogels increase from 45.9 to 70.3 mJ/m2 {after} plasma treatment.Compared with the untreated sample,BMSCs spread out more filopodia after 24 h incubation on treated films,and it enter cell division phase more quickly.BMSCs cultivation studies show that cells grow better on the plasma treated surface,which is beneficial to its cell compatibility.