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巴豆醛诱导人支气管上皮细胞凋亡研究 被引量：5

Caspase-mediated Apoptosis of Human Bronchial Epithelial Cells Induced by Crotonaldehyde
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摘要 为了评价卷烟烟气的危害性,研究了巴豆醛导致人支气管上皮细胞BEAS-2B死亡机制。即采用MTT法检测细胞存活率,化学发光法检测胞内ATP含量,流式细胞仪检测细胞凋亡率,流式细胞仪结合免疫化学检测线粒体细胞色素c,化学发光法检测caspase-9,caspase-3/7。结果显示,巴豆醛诱导细胞凋亡,且随着剂量增大,细胞由凋亡转向坏死。巴豆醛导致胞内ATP水平迅速下降,导致细胞线粒体膜电位下降,导致细胞色素c由线粒体释放至胞浆,激活caspase-9和caspase-3/7。结论:巴豆醛诱导细胞凋亡,且caspase级联酶参与巴豆醛诱导的细胞凋亡。 In order to assess the health risk of cigarette smoke,crotonaldehydeinduced cell death and its mechanism in BEAS-2B cells were investigated.Cell survival rate,intracellular ATP level,apoptosis rate,cytochrome c released from mitochondria to cytoplasm,and caspase-9 and caspase-3/7 activities were determined by MTT,chemiluminescence,flow cytometry,flow cytometry combined with immunochemistry,and chemiluminescence methods,respectively.The results showed that crotonaldehyde induced cell apoptosis,and turned into necrosis along with the increase of crotonaldehyde dosage.Crotonaldehyde caused that intracellular ATP level declined rapidly,mitochondria membrane potential decreased,cytochrome c released from mitochondria to cytoplasm,and caspase-9 and caspase-3/7 were activated.It was concluded that crotonaldehyde induced and caspase mediated cell apoptosis.
作者 刘兴余 杨陟华 潘秀颉 朱茂祥 谢剑平
机构地区 中国科学院大连化学物理研究所 上海烟草(集团)公司技术中心北京工作站 军事医学科学院放射与辐射医学研究所 中国烟草总公司郑州烟草研究院
出处 《烟草科技》 EI CAS 北大核心 2011年第1期36-42,共7页 Tobacco Science & Technology
关键词 巴豆醛 凋亡 CASPASE Crotonaldehyde Apoptosis Caspase
分类号 TS416 [农业科学—烟草工业]
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参考文献25

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2011年 第1期

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