三种不同部位移植胰岛细胞治疗糖尿病

Allograft pancreatic islets in three sites for treating diabetic rats

目的 对比通过肝脏、静脉、胰腺三种途径移植胰岛细胞对糖尿病大鼠的治疗作用.方法 24只糖尿病大鼠模型随机分为A、B、C组,A组在肝脏被膜下多点移植1000个胰岛细胞,B组通过尾静脉移植1000个胰岛细胞,C组在胰腺被膜下移植1000个胰岛细胞,于不同时间点测定大鼠随机血糖,对比大鼠血糖变化趋势及维持正常的时间.结果 A组大鼠血精于术后3 d内开始下降,血糖可降至正常水平（7.98±2.28）mmol/L,血糖维持正常水平（3.71±0.95）d,B组移植后24 h 血糖降至正常水平（7.35±1.40）mmol/L,可维持（7.85±1.46）d,C组移植后24 h血糖降至正常水平（7.06±2.11）mmol/L,可维持（24.90±2.60）d,不同部位移植对大鼠血糖水平变化的影响不同,差异有统计学意义（P＜0.05）.结论 在胰腺被膜下移植胰岛细胞血糖维持正常时间最长,是一个较理想的移植部位.

Objective To study the curative effectiveness of pancreatic islets transplantation through the liver, tail vein and pancreas. Methods Twenty-four diabetic rats were randomly divided into groups 1,2, 3. The rats in group Ⅰ were transplanted with 1000 pancreatic islets beneath the liver capsule,those in group 2 with 1000 pancreatic islets through tail vein, and those in group 3 with 1000 pancreatic islets beneath the pancreas capsule. Plasma glucose levels at different time points were determined and compared. Results In group 1, plasma glucose levels were reduced at the 3rd day post-transplantation,reached the normal level （7.98 ±2. 28） mmol/L and maintain （3.71 ±0. 95） days. Group 2 start to activate after 24 h. Plasma glucose level reach to （7.35 ± 1.40） mmol/L and maintain （7.85 ± 1.46） days.Group 3 start to activate after 24 h. Plasma glucose level reach to （ 7.06 ± 2. 11 ） mmol/L and maintain （24. 90 ± 2. 60 ） days. There is statistical significance in plasma glucose level and maintain normal time after transplantation pancreatic islet in different sites （ P 〈 0. 05 ）. Conclusion Transplantation pancreatic islets beneath the pancreas capsule maintain plasma glucose for the longest time. The pancreas is a ideal transplantation site.