低温保存不同时间大鼠带瓣管道活性与bax、bcl-2基因表达的关系

Effects of vascular cryopreservation time on the expression of bax and bcl-2 genes in rat valved conduit

目的 观察低温保存不同时间大鼠带瓣血管活性及bax和bcl-2基因的表达,探讨基因检测是否能成为带瓣血管活性指标.方法 Wistar大鼠80只,体质量250～350 g,分为新鲜组A组,-196 ℃液氮冻存3、6、9个月为B、C、D 3组.检测细胞结构、糖代谢及bax、bcl-2基因的变化.结果 大鼠带瓣血管的各组葡萄糖代谢率测定,A组（4.365±0.784）与B（4.383±0.548）、C组（4.446±0.608）、D组（4.090±0.657）差异无统计学意义（P＞0.05）;bax基因的表达分别为20%、45%、60%和85%,差异有统计学意义（P＜0.05）;bcl-2基因表达分别25%、30%、20%和35%,差异无统计学意义（P＞0.05）.结论 经冻存后的大鼠带瓣血管活性良好,提示bax基因可作为检测带瓣血管活性的指标,bcl-2的表达或高表达可能是细胞能耐受冻存打击的原因之一.

Objective To study the expression of bax and bcl-2 genes and activity of valved conduit in rats by cryopreservation for different lengths, and to probe whether genetic detection will become one of the indexes of valved conduit activity. Methods Eighty Wistar rats were divided into four groups： 20cases of fresh blood vessels （ group A ）, 20 cases of frozen vessels for 3 months （ group B ）, 20 for 6months （ group C）, and 20 for 9 months （ group D）. The changes in cellular structure, gluoce metabolism and the expression of bax and bcl-2 proteins were observed. Results The glucose metabolism rate of the rate valved conduit in groups A, B, C and D was 4. 365 ± 0. 784, 4. 383 ± 0. 548, 4. 446 ± 0. 608, and 4. 090 ± 0. 657 respectively, with the difference being not significant （ P 〉 0. 05 ）. The positive expression rate of bax protein in groups A, B, C and D was 20%, 45%, 60% and 85% respectively （P〈0. 05）.The positive expression rate of bcl-2 in groups A, B, C and D was 25%, 30%, 20% and 35% respectively （ P 〉 0. 05 ）. Conclusion The frozen rat valved vascular showed good activity. bax gene can be regarded as one of the indicators for detecting valved vessel activity. The expression or over-expression of bcl-2gene may be one of the reasons why the cells can tolerate the frozen preservation.