期刊文献+

聚(N-异丙基丙烯酰胺)-b-聚苯乙烯改性玻璃表面对细胞黏附/脱附的调控

Regulation of Cell Adhesion/Desorption by the Poly(N-isopropylacrylamide)-block-Polystyrene Modified Glass Surfaces
下载PDF
导出
摘要 通过表面引发连续原子转移自由基聚合的方法在玻璃表面接枝了聚(N-异丙基丙烯酰胺)-b-聚苯乙烯(PNIPAAm-b-PS)双嵌段聚合物刷。测试结果表明改性表面具有一定的浸润性温度响应性以及良好的生物学性能。与未改性的玻璃表面相比,改性后的表面具有良好的排斥血浆蛋白质的非特异性吸附能力。此外,PS链段的引入在不影响表面温敏性调控细胞黏附/脱附的条件下能够有效提高PNIPAAm改性表面在温度高于其低临界溶解温度(LCST)时对细胞的黏附能力。 Poly(N-isopropylacrylamide)-block-polystyrene(PNIPAAm -b-PS) brushes were prepared via surface-initiated consecutive atom-transfer radical polymerization on initiator-immobilized glass surfaces. The characterization results indicate that the modified surfaces exhibite thermo-responsivity of wettability and unique biological pro- perties. Compared with the unmodified glass surface, the PNIPAAm modified surface exhibits good non-specific protein resistance. Moreover, the introduction of PS block onto modified surfaces can enhance cell adhesion above the lower critical solution temperature(LCST) of PNIPAAm without affecting the thermo-responsive reversible adhesion and detachment of cells.
出处 《材料导报》 EI CAS CSCD 北大核心 2011年第2期23-26,共4页 Materials Reports
基金 国家自然科学基金(20974086)
关键词 聚(N-异丙基丙烯酰胺) 聚苯乙烯 嵌段共聚物 蛋白质吸附 细胞黏附 poly(N-isopropylacrylamide), polystyrene, block copolymer, protein adsorption, cell adhesion
  • 相关文献

参考文献16

  • 1Schild H G. Poly (N-isopropylacrylamide) :Experiment, theory and application[J]. Prog Polym Sci, 1992, 17 (2) : 16a.
  • 2Dimitrov I, Trzebicka B, Muller A H E, et al. Thermosensitive water-soluble copolymers with doubly responsive reversibly interacting entities [J]. Prog Polym Sci, 2007, 32 (11) :1275.
  • 3Okano T, Yamada N, Okuhara M, et al. Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces [J]. Biomaterials, 1995,16(4) : 297.
  • 4Matsuda N, Shimizu T, Yamato M, et al. Tissue engineering based on cell sheet technology[J]. Adv Mater,2007,19 (20):3089.
  • 5Akiyama Y, Kikuchi A, Yamato M, et al. Ultrathin poly (N-isopropylaerylamide) grafted layer on polystyrene surfaces for cell adhesion/detachment control[J]. Langmuir, 2004,20(13) :5506.
  • 6Fukumori K, Akiyama Y, Yamato M, et al. Temperatureresponsive glass coverslips with an ultrathin poly(N-isopropylacrylamide) layer [J]. Acta Biomater, 2009,5 (1) : 470.
  • 7Mizutani A, Kikuchi A, Yamato M, et al. Preparation of thermoresponsive polymer brush surfaces and their interaction with cells [J]. Biomaterials, 2008,29 (13) : 2073.
  • 8Li L, Zhu Y, Li B, et al. Fabrication of thermoresponsive polymer gradients for study of cell adhesion and detachment [J]. Langmuir,2008,24(23) :13632.
  • 9Yu Q, Zhang Y, Chen H, et al. Protein adsorption on poly (N-isopropylaerylamide)-modified silicon surfaces: Effects of grafted layer thickness and protein size[J]. Colloids Surf, B,2010,76(2) :468.
  • 10Chen H, Zhang Y, Li D, et al. Surfaces having dual fibrinolyric and protein resistant properties by immobilization of lysine on polyurethane through a PEG spaeer[J]. J Biomed Mater Res A,2009,90(3):940.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部