珠美海棠叶片离体培养与植株再生

Plant regeneration from in vitro cultured leaves of Malus zumi

将珠美海棠试管苗叶片接种于附加NAA 0.3 mg·L-1和不同体积分数的6-BA(1.0～6.0 mg·L-1)的MS培养基中进行离体培养,结果表明,6-BA为1.0～3.0 mg·L-1的处理芽再生率为6.7%～31.8%,而根的再生率为26.7%～28.3%。6-BA为4.5和6.0 mg·L-1处理芽再生率分别高达80.0%和60.0%,根的再生率小于5%。继代培养60 d的珠美海棠试管苗叶片的芽再生率显著低于继代培养30 d的叶片。采用横切主脉的叶片切伤方式,其切口处芽的再生率及整个叶片芽的再生率均显著高于纵切主脉两侧叶片、横切主脉两侧叶片及横剪主脉两侧叶片等切伤方式。

The leaves from in vitro plantlets of Malus zumi were cultured in MS medium containing 0.3 mg·L-1 NAA and different concentrations of 6-BA（1.0～6.0 mg·L-1）.The results showed that the bud regeneration rate was from 6.7% to 31.8% while the root regeneration rate was from 26.7% to 28.3% when 6-BA concentration was from 1.0 to 3.0 mg·L-1.In the treatments with 4.5 and 6.0 mg·L-1 of 6-BA the bud regeneration rates were up to 80.0% and 60.0%,respectively.The bud regeneration rate of the leaves from Malus zumi plantlets sub-cultured for 60 days was obviously lower than that of the plantlet leaves sub-cultured for 30 days.The regeneration rates of the buds from both the cutting places and the whole leaf under the condition of cutting the primary vein crosswise were obviously higher than those under the condition of cutting the leaf blade beside the primary vein lengthwise without cutting the leaf edge or cutting the leaf blade beside the primary vein crosswise without or with cutting the leaf edge.