鱼中挥发酚4-氨基安替比林流动注射分析

Flow injection analysis of volatile phenol in fish with 4-amino antipyrine colorimetry

目的探讨用4-氨基安替比林流动注射分析测定鱼中挥发性酚。方法对4-氨基安替比林流动注射分析的线性范围、最低检出限量、加标回收率及相对标准偏差进行测定,在操作质量合格的前提下,检测广东省深圳市52份海鱼及42份淡水鱼。结果 4-氨基安替比林流动注射分析线性范围为0.001～0.04 mg/kg,最低检出限<0.001 mg/kg,加标回收率89.5%～102.9%,相对标准偏差1.3%～4.6%;海鱼酚平均质量浓度(0.184 mg/kg)明显高于淡水鱼(0.061 mg/kg)(P(0.05);2009年淡水鱼酚比2007年(0.049 mg/kg,萃取法)高24%,但差异无统计学意义。结论 4-氨基安替比林流动注射分析的高自动化、微量操作,提高了方法的灵敏度和稳定性,符合高效、节能要求。

Objective To investigate the detection of volatile phenol in fish by flow injection analysis with 4-amino antipyrine colorimetry.Methods The linear range,detection limit,recovery,and relative standard deviation of flow injection analysis for volatile phenol with 4-amino antipyrine colorimetry were tested,and 52 samples of marine fish and 42 samples of freshwater fish were detected.Results The linear range was 0.001-0.04mg/kg;the detection limit was less than 0.001 mg/kg;the recovery was 89.5%-102.9% and the relative standard deviation was 1.3-4.6%.The average concentration of volatile phenol in marine fish（0.184 mg/kg）was significantly higher than that of in freshwater fish（0.061 mg/kg）（P0.05）.The volatile phenol concentration of freshwater fish was 24% higher in 2009 than that of in 2007（0.049 mg/kg）.Conclusion The high automation and micro-operation improve the sensitivity and stability of flow injection analysis with 4-amino antipyrine colorimetry and the method is efficiency and energy saving.