新疆雪莲化学成分的分离与鉴定

Isolation and identification of chemical constituents from whole plant of Saussurea involucrata Kar.et kir.

目的对新疆雪莲的化学成分进行研究。方法采用硅胶、凝胶柱色谱和重结晶等分离方法对体积分数为75%的新疆雪莲乙醇溶液提取物进行成分分离,通过谱学分析方法结合理化性质进行结构鉴定。结果共分离得到8个化合物,分别鉴定为大苞雪莲内酯(involucratolactone,1)、11α,13-dihydrozaluzanin-C(2)、dihydrozaluzanin-C(3)、7β-羟基谷甾醇(7β-hydroxysitosterol,4)、7β-羟基豆甾醇(stigmast-5,22-dien-3β,7β-diol,5)、山奈酚(kaempferol,6)、高车前素(hispidulin,7)、粗毛豚草素(jaceosidin,8)。结论化合物3、5为首次从风毛菊属植物中分离得到,化合物2、4为首次从新疆雪莲植物中分离得到。

Objective To study the chemical constituents of the whole plant of Saussurea involucrata Kar. et kit. Methods The chemical constituents of 75 % ethanol extract of Saussurea involucrata Kar. et kir. were isolated by silica gel column chromatography, sephadex LH-20 column chromatography, together with recrystallization method, and the structures were identified by means of NMR spectral analysis and physico-chemical properties. Results Eight compounds were obtained and elucidated as involucratolactone （1）, 11 α, β-dihydrozaluzanin-C （ 2 ）, dihydrozaluzanin-C （ 3 ）, 7fl-hydroxysitosterol （ 4 ）, stigmast-5,22-dien-3β, 7β-diol （5）, kaempferol （ 6 ）, hispidulin （ 7 ）, jaceosidin （ 8 ）, respectively. Conclusions Compounds 2,4 are isolated from this plant for the first time, while, compounds 3 and 5 are isolated from the Saussurea genus for the first time.