棉铃虫对甲氧虫酰肼抗性机理的初步研究

Preliminary study on mechanism of resistance to methoxyfenozide in cotton bollworm,Helicoverpa armigera(Hübner)

在室内通过抗性汰选和敏感性反汰选,获得了对甲氧虫酰肼抗性指数相差为72.61倍的棉铃虫Helicoverpa amigera(Hübner)抗性种群和敏感种群。通过增效试验和离体酶活性测定表明:棉铃虫对甲氧虫酰肼产生的抗药性主要与其多功能氧化酶(MFO)、全酯酶和谷胱甘肽-S-转移酶(GSTs)活性的提高有关。

A methoxyfenozide-resistant population and a homologous susceptible population of Helicoverpa armigera（Hübner） were selected in the laboratory.The resistant population showed 72.61-fold resistance to methoxyfenozide compared with susceptible one.Resistant mechanisms of H.armigera to methoxyfenozide were preliminary studied by synergism assay using piperonyl butoxide,phosphate defoliant and diethyl maleate in vivo,and by enzyme activity assay for mixed function oxidases,general esterases,and glutathione-S-transferases in vitro.The results showed that enhancement of activity of the three enzymes may contribute to methoxyfenozide resistance in the resistant population of H.armigera.