期刊文献+

促吸收剂癸酸钠对黄连素在大鼠肠道吸收的促进作用 被引量:4

Absorption enhancement of sodium caprate on berberine in intestine of rats
下载PDF
导出
摘要 目的:探讨黄连素(Ber)在大鼠肠道的吸收动力学,阐明癸酸钠(SC)对Ber在小肠段吸收的影响。方法:雄性Wistar大鼠建立在体肠段灌流模型,HPLC法测定不同浓度Ber(50、100和200μmol.L-1)和Ber与质量浓度为0.2%SC合用在小肠的吸收情况;测定肠灌流液中乳酸脱氢酶(LDH)活性,观察SC对肠道黏膜细胞的损伤。结果:Ber浓度在50~200μmol.L-1范围内Ber吸收呈浓度依赖性;吸收速率常数(Ka)组间差异无统计学意义(P>0.05);SC能显著提高Ber在小肠的吸收量(P<0.05);SC对Ber吸收速率常数Ka无显著影响(P>0.05);Ber组、SC+Ber组肠循环液中LDH的含量与空白对照组比较差异无统计学意义(P>0.05)。结论:Ber在小肠吸收较差,吸收机制为被动扩散,吸收过程为一级动力学过程;SC能显著促进Ber在小肠的吸收,是一种安全、有效的促吸收剂。 Objective To observe the intestine absorption kinetics of berberine(Ber) in rats and the effects of sodium caprate(SC) on Ber in intestinal absorption.Methods Male Wistar rats were used for the experiment.The in situ circular perfusion model was used to investigate the effect of SC on the intestinal absorption of Ber.The concentration of Ber was detected with HPLC.The activity of LDH was measured to evaluate the toxic effect of SC on mucous membrane cells.Results The absorption of Ber in the small intestine was poor.The uptake of Ber presented dose-dependent manner.There was no significant difference on Ka among these groups(P0.05).SC could significantly enhance the absorption amount of Ber in the small intestine(P0.05).SC showed no significant effect on Ka of Ber(P0.05).The LDH activities in circulating fluid in Ber and SC+Ber groups had no significant difference compared with control group(P0.05).Conclusion The absorption of Ber in the small intestine is poor and complied with the passive diffusion mechanism and first order kinetics.SC can significantly enhance the absorption of Ber in the small intestine and is a safe,effective absorption accelerator.
出处 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2011年第1期35-40,共6页 Journal of Jilin University:Medicine Edition
基金 吉林省科技厅高技术产业发展项目资助课题(2009633) 吉林大学大学生创新性实验计划项目资助课题(2008A71041)
关键词 癸酸钠 黄连素 大鼠 Wistar 小肠 吸收动力学 sodium caprate berberine rats Wistar intestine absorption kinetics
  • 相关文献

参考文献3

二级参考文献9

共引文献78

同被引文献59

  • 1Quangang Zhu,Zhongjian Chen,Pijush Kumar Paul,Yi Lu,Wei Wu,Jianping Qi.Oral delivery of proteins and peptides:Challenges, status quo and future perspectives[J].Acta Pharmaceutica Sinica B,2021,11(8):2416-2448. 被引量:18
  • 2曾美怡,李敏民,赵秀文.国外有关小檗碱毒性反应的报道[J].中药新药与临床药理,1995,6(3):47-48. 被引量:8
  • 3赵娜,郭治昕,赵雪,赵利斌.丹参的化学成分与药理作用[J].国外医药(植物药分册),2007,22(4):155-160. 被引量:258
  • 4Shantha KT, Chawla S, Nachaegari SK,et al. Validated HPLC analytical method with programmed wavelength UV detection for simultaneous determination of DRF-4367 and Phenol red in rat in situ intestinal perfusion study[ J]. J Pharm Biomed Anal ,2005,38 ( 1 ) : 173-179.
  • 5Stewart BH, Chan OH, Lu RH, et al. Comparison of intestinal permeabilities determined in multiple in vitro and in situ models:relationship to absorption in humans [ J ]. Pharm Res, 1995,12 ( 5 ) : 693-699.
  • 6Fagerholm U, Johansson M, Lennernas H. Comparison between permeability coefficients in rat and human jejunum[ J]. Pharm Res, 1996, 13(9) :1336-1342.
  • 7Li H, Zhao X, Ma Y, et al. Enhancement of gastrointestinal absorption of quercetin by solid lipid nanoparticles [ J ]. J Control Release ,2009, 133 (3) :238-244.
  • 8Yao G, Li Y. Preparation, characterization and evaluation of self-microemulsifying drug delivery systems (SMEDDSs) of Ligusticum chuanxiong oil[ J ]. Biomedicine & Pharmacotherapy,2010, on line.
  • 9Maher S, Wang X, Bzik V, et al. Evaluation of intestinal absorption and mucosal toxicity using two promoters. II. Rat instillation and perfusion studies[J]. Eur J Pharm Sci,2009,38(4) :301-311.
  • 10Zhou YQ,Li WZ, Chen LY, et al. Enhancement of intestinal absorption of akebia saponin D by bomeol and probenecid in situ and in vitro [ J ]. Environmental Toxicology and Pharmacology,2010,29 ( 3 ) : 229 - 234.

引证文献4

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部