PDGF家族在原发性胃腺癌组织中的表达及其意义

Expressions of platelet-derived growth factors in primary adenocarcinoma of stomach tissues and significance

目的:研究血小板衍生生长因子(PDGFs)家族在原发性胃腺癌组织中的表达,阐明其与原发性胃腺癌临床病理特征的关系。方法:选择手术切除并经病理证实的58例原发性胃腺癌癌组织及其对应的癌旁组织标本,采用RT-PCR方法检测组织中PDGF-A、B、C、D mRNA的表达情况,明确PDGF家族在mRNA水平的表达谱;免疫组织化学方法检测家族中重要成员PDGF-D蛋白的表达情况。结果:胃腺癌组织中PDGF-A、B、C、D mRNA阳性表达率分别为39.66%(23/58)、22.41%(13/58)、31.03%(18/58)和98.28%(57/58),与其相对应的癌旁组织的阳性表达率分别为12.07%(7/58)、10.34%(6/58)、17.24%(10/58)和72.41%(42/58),在胃腺癌组织中PDGF-D mRNA阳性表达率显著高于PDGF其他亚型(P<0.01),且PDGF-D mRNA的表达率显著高于癌旁正常组织(P<0.05);PDGF-D蛋白在胞膜/胞质均有表达;有淋巴结转移、远处转移及复发的胃腺癌组织PDGF-D mRNA的表达水平高于无转移或无复发组织(P<0.05)。结论:PDGF-D是PDGF家族中最重要的与胃腺癌关系密切的因子,提示检测PDGF-D的表达可以作为判断胃腺癌转移和预后的指标之一。

Objective To investigate the expressions of platelet-derived growth factors（PDGFs） in primary adenocarcinoma of stomach tissues,and clarify their relationships with clinicolpathological features of primary adenocarcinoma of stomach.Methods The mRNA expressions of PDGF-A,B,C,D were assessed by reverse transcription-polymerase chain reaction（RT-PCR）in 58 cases of gastric cancer and adjacent carcinoma tissues.Immunohistochemical staining was used to examine the expression of PDGF-D protein in gastric cancer and adjacent normal tissues.Results The positive expression rates of PDGF-A,B,C,D mRNA were 39.66%,22.41%,31.03%,and 98.28% in gastric cancer tissues and 12.07%,10.34%,17.24%,and 72.41% in adjacent normal tissues,respectively.The positive expression rate of PDGF-D mRNA in gastric cancer tissues was significantly higher than that in adjacent normal tissues（P0.05）.The expression of PDGF-D mRNA was significantly higher than other PDGF mumbers in gastric cancer tissues（P0.01）.The PDGF-D protein was present in membrane/ cytoplasm and the difference between gastric cancer and adjacent normal tissue was statistically significant（P0.05）.The PDGF-D mRNA expressions in groups with lymph-node metastasis,distant metastasis and relapse were higher than those in the groups without metastasis and relapse（P0.05）.Conclusion PDGF-D is the most important mumber of PDGFs and has a close relationship with human gastric cancer.It can be used as a prognostic marker of gastric cancer in clinical practice.