氟化钠对小鼠几种血清酶活性的影响

Effects of NaF on the activities of serum enzymes of mice

让刚断奶的小白鼠饮用NaF水溶液一个月后,测定其四种血清酶活性。在高氟条件下,血清中的谷草转氨酶、乳酸脱氢酶、羟丁酸脱氢酶的活力显著增高,而肌酸激酶的活力显著降低。小鼠肝和肾对氟中毒较为敏感,而骨骼肌和心肌对氟有较强的耐受力。对以上几种血清酶活性的测定可作为诊断氟中毒的一个辅助手段。

Objective to provide theoretical foundation and experimental method for diagnosising fluorosis through evaluating the activities of some important serum enzymes. Methods: Weanling mice were fed the drinking water containing 200mg/L, 400mg/L or 800mg/L NaF for a month, then the activities of glutamic-oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), hydroxybuyrae dehydrogenase (HBDH) and creatine kinase (CK) were determined. Results: The high NaF group had significatly higher activities of serum GOT, LDH and HBDH, but markedly lower serum CK activity. Conclusion The liver and kidney of mice are more sensitive than heart and skeletal muscle to fluorosis, and assaying the above serum enzyme parameters may be a supplementary method to diagnosis fluorosis.