摘要
为了确定应用颅骨外冷冻方法造成大鼠脑冻伤，从而导致血管源性脑水肿的最佳温度及时间，选用了－ ６０、－ ８０ 及－ １９６℃三个冷冻温度及 １、２、３、４ｍ ｉｎ 四个冷冻时间冷冻大鼠一侧大脑。结果发现采用－ １９６℃冷冻１ｍ ｉｎ 的脑冻伤模型大鼠脑水肿明显，此法操作简单，动物死亡率低。
In order to find out the most suitable treatment temperature and time for establishing a rat model of coldinduced vasogenic brain edema,three temperatures (-60℃、-80℃ and -196℃) and four time(1、2、3、4 minutes) were used.The brass cup,filled with acetone,at the temperature of -60℃、-80℃ and -196℃ was put directly on one side of the rat bony skull resoectuvekt.The results of microscopic and electromicroscopic examinantions revealed that frost at -196℃ for 1 minute.can induce rat brain edema obviously and easily with low mortality.
出处
《上海实验动物科学》
1999年第3期149-151,169,共4页
Shanghai Laboratory Animal Science
关键词
大鼠
脑冻伤
疾病模型
Rat Brain cold injury Vasogenic brain edema
