摘要
以重瓣大岩桐叶片为外植体,经秋水仙碱处理得到大量的多倍体植株。在培养基中加入秋水仙碱20mgL-1处理一周,可使重瓣大岩桐的诱变率达到62.5%.对再生植株进行形态学观察表明,多倍体植株比二倍体的茎粗壮,叶片增大,加厚。细胞学鉴定四倍体染色体数为2n=4x=52而二倍体的染色体数为2n=26形态学观察和细胞学观察有很好的符合性。对多倍体植株进行过氧化物同工酶分析表明,多倍体植株的同工酶酶谱与二倍体有很大的差异,在四倍体的酶谱中观察到了两条新谱带。
The polyploid plants were obtained from the leaves of Sinningia Speciosa (Lodd.)Hiern treated by colchicine. Best result for inducing tetraploid was by adding colchicine20 mg L-1 intothe medium treated for one week rather than by colchicine soaking. Morpho-logical observation showed that the sterns and the leaves of tetraploid plants were respectivelythicker and lalger than the diploid ones. The chromosome number of tetraploid plants was2n=4x=52, while that of diploid plants was 2n=26. Peroxidase isoenzyme analysis showedthat the enzyme activities between the polyploid and the diploid plants were quite different.Two new bands were observed in the isoenzyme map of tetraploid plants.
出处
《热带亚热带植物学报》
CAS
CSCD
1999年第3期237-242,共6页
Journal of Tropical and Subtropical Botany
关键词
大岩桐
秋水仙碱
多倍体
同工酶
Sinningia speciosa,Colchicine,Polyploid,Isoenzyme