美洲斑潜蝇实验种群的饲养技术

The mass rearing method for experimental population of Liriomyza sativae Blanchard

本文阐述了寄主植物的室内培养及美洲斑潜蝇的饲养技术。实验以花斑芸豆作为寄主植物。经试验，去除复叶对增加真叶有效利用面积有一定作用。经１０天左右的培养，叶片长、宽度增长速率明显减慢，此时是接种斑潜蝇的最佳时机，１５天后即斑潜蝇幼虫生长到３龄时，叶面积达最大。在２５℃室温下，１４小时光照，６０％的相对湿度，２只接种箱，每天加入成虫１００头（雌：雄＝１：１），并更换新苗１０～１２盆，共６０～７２片叶子，生长期１０～１５天，每片叶子的面积可达２５～４０ｃｍ。照此每天可得６００～１０００头蛹即可基本满足室内工作的需要。

The study developed a method, to mass rear experimental population of L. sativae in laboratory. The results showed that the color bean Phaseolus vulgaris L. was the best host for the leafminer ovipositing and completing its life history. The increasing rate of width and length of primary leaves decreased obviously after 10 days planting. The leaves got their maximum areas after 15 days while L. sative was just at the 3nd instar stage. On the condition of 25℃ tem- perature,60% RH and 14: 10(L: D)h,relaesing 100 adults( : = 1: l) into the cage with 10 - 12 pots of host pla- nts,600 - 1000 pupae could be obtained everyday to meet the research need.