痘苗病毒增强子样序列的特性研究

STUDY OF THE CHARACTERISTICS OF VACCINIA VIRUS ENHANCER-LIKE ELEMENT

利用带有氯霉素乙酰转移酶报道基因的检测载体，从痘苗病毒基因组 Ｄ Ｎ Ａ 中筛选到一个增强子样片段。序列分析表明，该片段长２８３ｂｐ ，是痘苗病毒 Ｄ Ｎ Ａ 依赖性 Ｒ Ｎ Ａ 聚合酶的一部分，具有两段２４ｂｐ 长的重复序列。采用带有β－ 半乳糖甘酶报道基因的载体检测发现，该片段正向可以使报道基因表达增强１０９ 倍，反向可以增强３８ 倍。 Ｒ Ｎ Ａ ｄｏｔ ｂｌｏｔ 实验证实，它对基因的增强活性表现在转录水平上。

An enhancer-like element of 283bp from vaccinia virus genome DNA was obtained by using the plasmid carrying CAT reporter gene. Sequence analysis showed the element is a part of the DNA-dependent RNA polymerase, it has two 24bp repeated sequences. Detection of β-galactosidase activity showed that both directions can increase the activity 10.9, 3.8 times, respectively. The enhancing activity of the element are on transcription level.