三氧化二砷诱导细胞凋亡对线粒体的影响

INFLUENCE OF APOPTOSIS INDUCED BY ARSENIC TRIOXIDEON MITOCHONDRION

线粒体作为细胞能量的“动力工厂”，在细胞的生理过程中起着重要的作用，本文采用三氧化二砷（Ａｓ２ Ｏ３） 诱导ＭＧＣ－ ８０３ 细胞凋亡，通过罗丹明１２３ 和Ｈｏｅｃｈｅｓｔ３３２５８ 对细胞进行荧光标记，利用具有光子计数成像能力的超高灵敏度荧光显微镜观察凋亡细胞与未加入Ａｓ２ Ｏ３ 的对照组细胞的区别，发现Ａｓ２ Ｏ３

Mitochondrion plays an important role in the cell physiological process. In this article, the change of apoptotic MGC-803 cell induced by As2O3 was investigated. The fluorescent probes used were Rhodamine 123 and Hoechest 33258. By the ultra-high-sensitive fluorescence microscope with the ability of photon counting, the characteristics of the apoptotic cells were compared with those without As2O3. The result indicated that the reduction of the electric potential differences of mitochondrial membrane was one of important roles of As2O3 inducing MGC-803 cells to apoptosis.