周期应变对动脉平滑肌细胞分泌血管紧张素II的影响

THE EFFECT OF MEMBRANE CYCLIC TENSION STRAIN ON ANGIOTENSⅡ SECRETION OF RAT AORTIC SMOOTH MUSCLE CELLS

应用自行设计的硅胶膜伸张加载装置对培养于硅胶膜上的Ｗｉｓｔａｒ 大鼠主动脉平滑肌细胞施以周期性二维伸张应变（ 最大伸长比：纵向０ ．７ ％ ，横向０ ．３ ％ ） ，运用放射免疫沉淀法对其ＡｇＩＩ 的分泌量进行测定， 结果表明： 加载组血管紧张素ＩＩ 分泌量明显高于对照组（Ｐ＜ ０ ．００１ ，２ｈ ，１０ｈ 除外） ，对照组分泌曲线较平坦，而加载组曲线较尖锐；加载４ｈ ，ＡｇＩＩ分泌量达到峰值（２１９ ±２０ ．０ｐｇ／１０５ｃｅｌｌｓ） 。结合形态观察。

Using our own designed membrane stretching device cyclic strain was applied to rat aortic smooth muscle cells (RASMCs) cultured on a silicone rubber membrane. The result shows that: the RASMCs in stretch group secret more angiotens II than that in control group(p<0.001). The curve of angiotens II secretion in control group appeared flatter,but in stretching group, the peak of which at 4h reached 219±20.0pg/105cells. Combined with the result of morphology observation, it is found that the mechanical signal would effect the behavior of aortic smooth muscle cells greatly.