摘要
用ＳＤＳＰＡＧＥ 电泳分析和生物测定方法研究了过氧化氢（Ｈ２Ｏ２） 和羟自由基（·ＯＨ）对苏云金芽孢杆菌（ Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ） 伴孢晶体的损伤作用。结果表明，这两种活性氧对伴孢晶体均有一定程度的损伤作用，这种损伤作用与活性氧的浓度成正相关，并且·ＯＨ 对伴孢晶体的损伤作用明显强于Ｈ２Ｏ２ 。
The effects of hydrogen peroxides(H2O2)and hydroxyl radicals(·OH)on the purified parasporalcrystalsfrom Bacillusthuringiensisvar. Kurstaki HD 1 werestudiedby SDS PAGEand bioassay.Theresultsshowed thatto some extent H2O2 and ·OHproduced a direct and deleterious effect. Hydroxylradicalsfrom the system Fe( Ⅱ) EDTA H2O2indicated more serious damagethan hydrogen peroxides. There was a 44-5 % loss in activity against 12 h old larvae of Heliothis armigera when parasporalcrystals weretreated with 0-3 % H2O2 only. However,85-2 % activity lost when parasporalcrystals weretreated with 0-3% H2O2in thepresenceofiron( Ⅱ) and EDTA.
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第5期469-474,共6页
Acta Microbiologica Sinica
关键词
过氧化氢
羟自由基
苏云金芽孢杆菌
伴孢晶体
Hydrogen peroxide, Hydroxylradical, Bacillus thuringiensis parasporal crystal
